中国现代医学杂志2018，Vol.28Issue(18)：24-28,5.DOI:10.3969/j.issn.1005-8982.2018.18.005
球周注射尼莫地平对兔慢性高眼压致视网膜神经节细胞凋亡的干预作用
Intervention effect of Nimodipine injected around eyeball of rabbit on retinal ganglion cell apoptosis caused by chronic intraocular hypertension
黄帅 1张新跃 1张雅馨 1刘颖 1李琰 1周亚男 1王林洪1
作者信息
- 1. 华北理工大学附属医院 眼科,河北 唐山 063000
- 折叠
摘要
Abstract
Objective To investigate the intervention effect of Nimodipine injected around eyeball of rabbit on retinal ganglion cell apoptosis caused by chronic intraocular hypertension. Methods Thirty healthy New-Zealand albino rabbits were randomly assigned to 5 groups. Group A is blank control group and the rest of the groups (B, C, D and E) were chronic intraocular hypertension (CIH) ones which were induced by compound Carbomer. Group B were injected by Saline Injection (SI). Group C, D and E were injected by Nimodipine with low (0.125 mg), medium (0.250 mg) and high (0.500 mg) dose, respectively. The models were executed at the 2nd week. The retinal tissues in the groups were detected by immunochemistry, TUNEL and HE to identify the differences. Result The expression level of Bcl-2 in group D and E were significantly higher than that in group B and C (P ＜ 0.05). The expression of Bax in group B and C had a relatively high expression,while the expression of positive cells was decreased with the increase of NMD dose. The expression levels of Bax in group D and E were lower than those in group B and C (P ＜0.05). With TUNEL method, the apoptosis expression in group D and E were significantly lower than that in group B and C (P ＜ 0.05). Conclusion Nimodipine injected around eyeball can protect the retinal ganglion cells caused by chronic intraocular hypertension.关键词
慢性高眼压/球周注射/视网膜神经节细胞/尼莫地平Key words
chronic intraocular hypertension/peribulbar injectiont/retinal ganglion cell/Nimodipine分类
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黄帅,张新跃,张雅馨,刘颖,李琰,周亚男,王林洪..球周注射尼莫地平对兔慢性高眼压致视网膜神经节细胞凋亡的干预作用[J].中国现代医学杂志,2018,28(18):24-28,5.
