中国现代医学杂志2018，Vol.28Issue(18)：29-33,5.DOI:10.3969/j.issn.1005-8982.2018.18.006
重组人可溶性血栓调节蛋白对小鼠脑缺血/再灌注损伤的神经保护作用及机制研究
Neuroprotective effect of ART-123 on ischemia / reperfusion injury in mice and its mechanism
黄山鉴 1韦贵宁 2姚人银 1邓小玲 1黄祖贵1
作者信息
- 1. 广西医科大学第十附属医院(钦州市第一人民医院)药剂科,广西 钦州 535000
- 2. 广西中药与天然药物工程技术研究中心,广西 南宁 530022
- 折叠
摘要
Abstract
Objective To investigated the effects of recombinant human soluble thrombomodulin alpha (ART-123) on cerebral ischemic/reperfusion injury in mouse model. Methods BALB/c mice were subjected to 4 h-middle cerebral artery suture occlusion (MCAO) and treated at reperfusion with Vehicle or ART-123 (1 or 5 mg/kg, i.v) after 4 h MCAO. Infarct volume, motor coordination, plasma high-mobility group box (HMGB1) level and hemorrhage volume were evaluated 24 h after 4 h MCAO. Results The infarct volume was reduced by ART-123 in mice subjected to 4 h MCAO in a dose-dependent manner (F = 4.843, P = 0.038). Moreover, ART-123 (5 mg/kg) improved motor coordination determined by the rotarod test (P = 0.028), and decreased plasma HMGB1 level compared with Vehicle-treated controls (P = 0.000). In addition, there was no difference in hemorrhage volume between Vehicle-treated control group and the ART-123 treatment group. Conclusions ART-123 may improve cerebral ischemia /reperfusion injury in mice by inhibiting HMGB1.关键词
重组人可溶性血栓调节蛋白/缺血/再灌注损伤/小鼠Key words
ART-123/ischemic/reperfusion injury/mice分类
医药卫生引用本文复制引用
黄山鉴,韦贵宁,姚人银,邓小玲,黄祖贵..重组人可溶性血栓调节蛋白对小鼠脑缺血/再灌注损伤的神经保护作用及机制研究[J].中国现代医学杂志,2018,28(18):29-33,5.
