中国现代医学杂志2018，Vol.28Issue(18)：34-38,5.DOI:10.3969/j.issn.1005-8982.2018.18.007
六价铬对大鼠学习记忆的影响
Effect of chromium exposure on rats' learning and memory ability
黄国兴 1高琳 1赵丹婷 2杨文强 2柳晓琳 1杨菁2
作者信息
- 1. 锦州医科大学 公共卫生学院,辽宁 锦州 121001
- 2. 锦州医科大学 辽宁省心脑血管药物基础研究重点实验室,辽宁 锦州 121001
- 折叠
摘要
Abstract
Objective To investigate the effects of chromium exposure on learning and memory in rat model. Methods The rats were fed with distilled water (control group) or different concentrations of potassium bichromate solution (0.8, 4.0, 20.0 mg/kg) during lactation period. After weaning, the offspring rats were directly fed with the solutions the same as their mother rats for 6 months. The content of chromium in cerebral tissue were determined with the graphite furnace atomic absorption spectrophotometer. Learning and memory abihties of rats were tested by the Morris water maze experiment. The Nissl staining and immunohistochemical assay were performed to observe the morphological changes of the rat hippocampal nerve cells and astrocytes. High performance liquid chromatography was utilized to determine the content of glutathione (GSH). Results Chromium content in cerebral tissue of rats receiving various concentrations of potassium dichromate increased significantly compared with control group (P ＜0.05). The time of passing through the platform and staying around the platform in potassium dichromate exposure group were prolonged compared with control group. With the increase of exposure dose, The average latency and latency to first entry were increased while number of animals crossing original platform and time of stay in the primary quadrant were decreased in dose dependent manner in exposure group when compared with those in control group (P ＜ 0.05). Compared with the control group, potassium bichromateinduced hippocampus damage including disordered neurons, and concentrated and contracted nucleus. Expression of GFAP in astrocytes increased while GSH content in hippocampus tissue decreased in exposure group in dose dependent manner when compared with control group (P ＜ 0.05). Conclusion Exposure to chromium causes disfunction of hippocampal neurons, compromising learning and memory ability.关键词
六价铬/学习记忆/神经元/星形胶质细胞/谷胱甘肽Key words
hexavalent chromium/learning and memory/neuron/astrocyte/glutathione分类
医药卫生引用本文复制引用
黄国兴,高琳,赵丹婷,杨文强,柳晓琳,杨菁..六价铬对大鼠学习记忆的影响[J].中国现代医学杂志,2018,28(18):34-38,5.
