中国现代医学杂志2018，Vol.28Issue(20)：1-5,5.DOI:10.3969/j.issn.1005-8982.2018.20.001
VEGF、VEGFR2在慢性砷中毒大鼠生精小管中的表达及其意义
Expressions of VEGF and VEGFR2 proteins in seminiferous tubules of rats with chronic arsenic poisoning
摘要
Abstract
Objective?To study the expressions of VEGF and its receptor 2 (VEGFR2) in the seminiferous tubules of chronic arsenic poisoning rats, to explore their relationships with the male infertility and the mechanism of male sterility.?Methods?Forty male Sprague-Dawley rats were randomly divided into four groups, i.e. high-dose arsenic exposure group (60.0 mg/L), medium-dose arsenic exposure group (12.0 mg/L), low-dose arsenic exposure group (2.4 mg/L), and control group (distilled water). The rats were treated with arsenic through drinking water for 6 consecutive months. In the end of the experiment, expressions of VEGF and VEGFR2 were detected by immunohistochemistry, the grey values of apoptosis cells in the seminiferous tubular epithelia of the rats were determinated by TUNEL method, the sperm morphology and sperm motility were observed.?Results?Immunohistochemical results showed that the expression levels of VEGF and VEGFR2 in the arsenic exposure groups were significantly lower than those in the control group (P < 0.05). TUNEL method results showed that compared with the control group, the grey values of apoptosis cells in the seminiferous tubular epithelia of the rats obviously decreased in the arsenic exposure groups (P < 0.05); with the increase of arsenic exposure dose, the grey values of apoptosis cells in the seminiferous tubules showed a trend of decrease. Compared with the control group, the number of normal sperms decreased, the sperm deformity rate increased and the sperm motility dropped in the arsenic exposure groups (P < 0.05).?Conclusions?Chronic arsenic poisoning affects expressions of VEGF and VEGFR2 in seminiferous tubules of rat testis and can cause apoptosis of seminiferous epithelial cells, resulting in male infertility.关键词
血管内皮生长因子/血管内皮细胞生长因子受体2/砷中毒/生精小管Key words
VEGF/VEGFR2/arsenic poisoning/seminiferous tubule分类
医药卫生引用本文复制引用
戴研平,王平,高晓勤..VEGF、VEGFR2在慢性砷中毒大鼠生精小管中的表达及其意义[J].中国现代医学杂志,2018,28(20):1-5,5.基金项目
贵州省科学技术基金[No :黔科合人才团队(2014)4005号] （2014）
贵州省遵义市科技计划[No :遵市科合设字(2014)01号] （2014）
