中国现代医学杂志2018，Vol.28Issue(20)：6-13,8.DOI:10.3969/j.issn.1005-8982.2018.20.002
雷公藤内酯醇抑制TNF-α表达对小鼠溃疡性结肠炎的影响
Effect of inhibition of TNF-α expression by triptolide on experimental ulcerative colitis in mice
摘要
Abstract
Objective?To investigate the effect of triptolide (TL) on ulcerative colitis (UC) and explore the potential association between therapeutic effect of TL and TNF-α expression using dextran sulfate sodium salt (DSS) mouse model.?Methods?The DSS mouse model was established to simulate human UC. Eighty BALB/c female mice were randomly allocated into eight groups with equal number. Group A was blank control group, the mice in group B received normal saline injection, the mice in group C received propylene glycol injection, the mice in group D was injected with Dexamethasone, the mice in group E was fed with water-dissolved Mesalazine through stomach tube at a daily dose of 20-30 mg/kg, and the mice in groups F, G and H were administered with different doses of TL (TL1, TL2 and TL3). Information regarding mice activity, diet and stool property was recorded daily. The mice were sacrificed on day 8, and disease activity index, colon tissue histological score, and colon pathological score were calculated. The serum level of TNF-α was tested by ELISA, and TNF-α expressions in the colonic mucosa specimens were examined by fluorescence quantitative PCR at gene level and by Western blot at protein level.?Results?In the TL (TL2 and TL3), Mesalazine and Dexamethasone-treated mice, the symptoms of colitis were relieved, TNF-α expression was significantly decreased compared with the normal saline and propylene glycol injection groups (P < 0.05).?Conclusions?TNF-α expression is upregulated in UC mouse model and involved in the development and progression of UC. TL can inhibit TNF-α expression, suggesting that TL may be a novel therapeutic drug for the treatment of UC.关键词
溃疡性结肠炎/肿瘤坏死因子-α/雷公藤内酯醇/治疗/动物实验Key words
ulcerative colitis/ TNF-α/ treatment/ triptolide/ animal experiment分类
医药卫生引用本文复制引用
张海峰,张彬,周国雄,陈卫昌..雷公藤内酯醇抑制TNF-α表达对小鼠溃疡性结肠炎的影响[J].中国现代医学杂志,2018,28(20):6-13,8.基金项目
江苏省南通市社会发展基金(No :BK2012074) （No :BK2012074）
