中国现代医学杂志2018，Vol.28Issue(20)：14-19,6.DOI:10.3969/j.issn.1005-8982.2018.20.003
运动联合抗抑郁药物对大鼠海马细胞和BDNF/pERK信号通路的影响
Effect of exercise combined with antidepressants on hippocampal cells and BDNF/pERK signaling pathway in rats
包玲 1胡晓华 1王宗琴 1高作慧 1冯映映1
作者信息
- 1. 华中科技大学同济医学院附属武汉精神卫生中心 精神科,湖北 武汉 430022
- 折叠
摘要
Abstract
Objective?To study the antidepressive effect and mechanism of exercise combined with Paroxetine.?Methods?Using the method of chronic unpredictable mild stress (CUMS), depression rat model was prepared. The rats were divided into control group, model group, exercise group, Paroxetine group, and combination (exercise and Paroxetine) group. Through regular exercise and Paroxetine intervention, the behavior of the rats was detected by sugar water and Morris water maze test, and used as an objective indicator of lack of pleasure to determine whether the model was duplicated successfully or not. The apoptotic rate of hippocampal cells was detected using flow cytometry. The level of reactive oxygen species (ROS) was measured by DCFH-DA. The expression levels of brain-derived neurotrophic factor (BDNF), phosphorylated extracellular signal-regulated kinases (pERK) and neuropeptide (VGF) were detected using Western blot.?Results?Compared with the control group, the consumption of sugar water was significantly decreased and the escape latency was prolonged in the model group (P < 0.05). Compared with the exercise group, the consumption of sugar water significantly increased, the escape latency was significantly shortened in the combination group on the 21st d (P < 0.05). The ROS level and the apoptotic rate of the combination group were reduced compared to those of the exercise group (P < 0.05). The expressions of BDNF, pERK and VGF proteins in the hippocampal cells of the model group were significantly lower than those of the control group (P < 0.05). The expressions of BDNF, pERK and VGF proteins were significantly increased in the combination group compared with the exercise group (P < 0.05).?Conclusions?Paroxetine combined with exercise has better antidepressive effect than exercise therapy or drug therapy alone, can alleviate the lack of pleasure, and reduce oxidative stress injury and apoptosis rate in the hippocampal cells by regulating the expression of downstream target gene VGF through BDNF/pERK signaling pathway.关键词
运动/抑郁症/海马细胞/凋亡/BDNF/pERK信号通路Key words
exercise/depression/hippocampal cell/apoptosis/BDNF/pERK signaling pathway分类
医药卫生引用本文复制引用
包玲,胡晓华,王宗琴,高作慧,冯映映..运动联合抗抑郁药物对大鼠海马细胞和BDNF/pERK信号通路的影响[J].中国现代医学杂志,2018,28(20):14-19,6.
