中国现代医学杂志2018，Vol.28Issue(20)：20-27,8.DOI:10.3969/j.issn.1005-8982.2018.20.004
KLF5在脱氧胆酸诱导胃黏膜肠上皮化生中的作用
KLF5 promotes DCA-induced intestinal metaplasia of gastric mucosa by activating Wnt pathway
摘要
Abstract
Objective?To investigate the role of Krüppel-like factor 5 (KLF5) in the process of deoxycholic acid (DCA)-induced intestinal metaplasia of gastric mucosa and its potential mechanism.?Methods?The expressions of KLF5 and Wnt3a in GES-1 cells treated with different concentrations of DCA, normal gastric mucosa and intestinal metaplasia tissues were detected by Western blot and RT-PCR. GES-1 cells were divided into blank control group, DCA group, DCA+si-Cont group and DCA+si-KLF5 group. Then the cell proliferation ability and apoptosis were detected by MTT assay and flow cytometry, respectively. The expressions of TFF2, VIL1 and CDX2 were detected by Western blot and RT-PCR. Furthermore, the protein expression levels of Wnt3a, β-catenin and CDX2 in si-KLF5 transfected GES-1 cells were detected after treatment with LiCl (Wnt activator) by Western blot.?Results?The expression levels of KLF5 and Wnt3a were significantly increased in the intestinal metaplasia tissues compared with the normal gastric mucosa tissues, they were also increased gradually with the increase of DCA concentration in the GES-1 cells. In addition, the increased cell proliferation ability, the decreased cell apoptosis rate and the elevated expressions of TFF2, VIL1 and CDX2 were detected in the GES-1 cells after treatment with 500 μmol/L DCA. However, the cell proliferation was decreased, the cell apoptosis was increased and the expressions of TFF2, VIL1 and CDX2 were downregulated in the GES-1 cells after transfection with si-KLF5, which attenuated the effect of DCA. Furthermore, Wnt activator LiCl attenuated the effect of si-KLF5 on the expressions of Wnt3a, β-catenin and CDX2 in the DCA-induced cells.?Conclusions?KLF5 may promote the DCA-induced intestinal metaplasia of gastric mucosa by activating the Wnt pathway.关键词
Krüppel-Like Factor 5/脱氧胆酸/肠上皮化生/Wnt/β-cateninKey words
Krüppel-like factor 5/DCA/intestinal metaplasia/Wnt/β-catenin分类
医药卫生引用本文复制引用
孙杰,付立芳..KLF5在脱氧胆酸诱导胃黏膜肠上皮化生中的作用[J].中国现代医学杂志,2018,28(20):20-27,8.基金项目
山东省教育厅课题(No :J13LK56) （No :J13LK56）
山东省临沂市科技发展计划项目(No :201515053) （No :201515053）
