中国现代医学杂志2018，Vol.28Issue(20)：28-33,6.DOI:10.3969/j.issn.1005-8982.2018.20.005
β连环蛋白对脑胶质瘤辐射抗性的影响
Effect of β-catenin on radiation resistance of glioma
高力扬 1李锦宏 2陈兵 2杨帆 2岑学程 2廖壮槟 2龙霄翱 2王思捷3
作者信息
- 1. 宁夏大学 生命科学学院,宁夏 银川 750021
- 2. 广东医科大学附属医院 神经外科,广东 湛江 524001
- 3. 广东医科大学附属医院 临床研究中心,广东 湛江 524001
- 折叠
摘要
Abstract
Objective?To explore the effect of β-catenin on proliferation and survive of U87 glioma cells after radiation.?Methods?To reduce the accumulation of β-catenin, U87 glioma cells were treated with 5 μmol/L IWR-1-endo. CCK-8 and single colony formation assay were used to examine the proliferation and survival of U87 cells after radiation. And immunocytochemistry (ICC) was used to monitor the expressions of related proteins in the cells. Moreover, electron microscopy was used to observe the changes of microstructures of the cells.?Results?The expression of β-catenin decreased dramatically in the U87 cells after radiation treatment compared to the control group (P < 0.05). After radiation, the proliferation of the IWR-1-endo-treated cells and the plate colony-forming efficiency increased (P < 0.05).?Conclusions?Decreased accumulation of β-catenin might reduce the proliferation of glioma cells through inhibition of the Wnt/β-catenin signaling pathway, but it might cause radiation resistance of glioma cells by protecting the mitochondria of the cells.关键词
人脑胶质瘤/β-catenin/辐射抗性Key words
glioma/β-catenin/radiation resistance分类
医药卫生引用本文复制引用
高力扬,李锦宏,陈兵,杨帆,岑学程,廖壮槟,龙霄翱,王思捷..β连环蛋白对脑胶质瘤辐射抗性的影响[J].中国现代医学杂志,2018,28(20):28-33,6.
