中国药科大学学报2018，Vol.49Issue(3)：341-347,7.DOI:10.11665/j.issn.1000-5048.20180313
多种细胞自噬调节剂对自噬标记物LC3Ⅱ及p62表达的影响
Effects of various autophagy modulators on the expression of autophagic markers LC3ll and p62
摘要
Abstract
Western blotting of autophagic markers LC3Ⅱ and p62 are widely used for estimating autophagic activity. To compare the regulation of various autophagy modulators on LC3Ⅱ and p62,HEK293 cells were treated separately with mTOR-dependent autophagy activator rapamycin or -independent autophagy activators trehalose, and autophagy inhibitors including 3-methyladenine (3-MA),bafilomycin A1 or E64d and pepstatin A that inhi-bited the initiation of autophagy,the fusion of autophagosome and lysosome,and the activities of lysosomal enzymes accordingly,and then LC3Ⅱ and p62 levels were assessed. Western blot results demonstrated that rapam-ycin enhanced the conversion of LC3I to LC3Ⅱ,promoted the degradation of p62 simultaneously,while trehalose merely increased the expression of LC3Ⅱ with no influence on the p62 level. Moreover,inhibition of autophagy commonly led to accumulation of LC3Ⅱ as well as blockage of p62 degradation in a concentration- and time-dependent manner. These results indicate that obvious differences exist in the regulation of LC3Ⅱ and p62 by various modulators although both are autophagic markers.关键词
自噬活化剂/自噬抑制剂/LC3Ⅱ/p62蛋白Key words
autophagy activator/autophagy inhibitor/LC3Ⅱ/p62分类
医药卫生引用本文复制引用
魏砚明,任晋宏,栾智华,王永辉..多种细胞自噬调节剂对自噬标记物LC3Ⅱ及p62表达的影响[J].中国药科大学学报,2018,49(3):341-347,7.基金项目
山西省卫生计生委科研课题资助项目(No. 201601114) （No. 201601114）
山西中医药大学博士启动基金资助项目(No. 2015BK16)This study was supported by the Research Project of Shanxi Provincial Health and Family Planning Commission（No. 201601114）and the Scientific Research Fund for the Doctoral Young Scholars of Shanxi University of Chinese Medicine （No. 2015BK16）
