中国烟草科学2018，Vol.39Issue(3)：1-9,9.DOI:10.13496/j.issn.1007-5119.2018.03.001
烤烟品种大白筋599特征香气性状的物质及分子基础研究
Study on Key Components and Molecular Basis Responsible for the Specific Aroma Traits of Flue-cured Tobacco Dabaijin599
摘要
Abstract
The objective of this study was to explore the key aroma component and molecular basis responsible for the specific aroma traits of flue-cured tobacco Dabaijin599, which will lay the foundation for developing flue-cured tobacco varieties with specific aroma traits and industrial utilization of their leaves. Leaf superficial exudates of different tobacco varieties (lines) were detected and smoking evaluation of the corresponding tobacco leaves was conducted to determine the main aroma components contributed to the specific aroma traits of Dabaijin599. Sequence polymorphism analysis of the key genes regulating the aroma component predicted was conducted to explore the molecular mechanism of specific aroma traits of Dabaijin599. The results showed that leaf superficial exudates of flue-cured tobacco Dabaijin599 and Oriental tobacco Samsun contain labdanoid diterpene abienol, while other flue-cured tobacco variety G28 etc. have no labdanoid diterpene abienol. Among the backcross or hybridization population of G28 with Dabaijin599, the offsprings contained abienol present special aroma characteristics like Dabaijin599, however, the ones without abienol had no special aroma characteristics. The 292th base of the key gene NtCPS2 controlling the synthesis of abienol was the wild type G in Dabaijin599 and the mutation type T in common flue-cured tobacco cultivars. Abienol was the key aroma component contributed to specific aroma of flue-cured tobacco variety Dabaijin599. SNP polymorphism in the 292th base of the gene NtCPS2, among Dabaijin599 and other varieties, results in the aroma characteristic difference between Dabaijin599 and the common flue-cured tobacco varieties. Enriching the type and content of aroma components by genetic improvement is an effective way to breed flue-cured tobacco varieties with special aroma.关键词
大白筋599/特异香型烤烟/基因NtCPS2/冷杉醇Key words
Dabaijn599/flue-cured tobacco with specific aroma characteristics/gene NtCPS2/abienol分类
农业科技引用本文复制引用
常爱霞,胡日生,向世鹏,王元英,屈亚芳,王国平,李洋洋,罗成刚,冯全福,陈志强,付宪奎,杨爱国..烤烟品种大白筋599特征香气性状的物质及分子基础研究[J].中国烟草科学,2018,39(3):1-9,9.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC01) （ASTIP-TRIC01）
中国烟草总公司湖南省公司科技项目"烟草表面致香前体物分子模块创制及湖南主栽品种香气性状定向改良"(18-20Aa02) （18-20Aa02）
