中国烟草科学2018，Vol.39Issue(3)：10-17,8.DOI:10.13496/j.issn.1007-5119.2018.03.002
基于RAD重测序技术开发烟草品种SNP位点
Development of Tobacco SNPs based on RAD Re-sequencing Technology
摘要
Abstract
Single Nucleotide Polymorphisms (SNPs) are the most widely used molecular markers in the genomics field. In this study, high throughput SNPs were developed through re-sequencing of ten tobacco varieties using Restriction-site Associated DNA (RAD) technology. The sequencing clean data of 44.33 Gb was obtained, with an average genome coverage of 1.01 X. 291,770 SNPs were identified and the average spacing between adjacent SNPs was 10.066±29.801 kb. The SNPs cover the whole genome, but the distribution density of SNPs varies with the location of chromosomes. There is a long range of SNP-dense area in the upper arm of the 17th chromosome. Functional annotation showed that transition is the main type of SNP mutation and 45,049 SNPs were found in the gene regions. Based on the SNPs population genotyping information, genetic distance between the tested varieties was calculated, and the results showed that the average genetic distance is 0.29 and the genetic distance between Taiyan 8 and other varieties is the farthest. The results will provide scientific basis for tobacco QTL mapping, candidate gene discovery and parental group matching.关键词
烟草/限制性内切酶位点标签/重测序/单核苷酸多态性Key words
Nicotiana tabacum L./restriction-site associated DNA/re-sequencing/single nucleotide polymorphism分类
农业科技引用本文复制引用
任民,程立锐,刘旦,蒋彩虹,杨爱国..基于RAD重测序技术开发烟草品种SNP位点[J].中国烟草科学,2018,39(3):10-17,8.基金项目
中国烟草总公司重大专项"以K326为主要底盘品种的烟草全基因组模块构建"[110201601028 (JY-02)]、"烟草抗CMV和赤星病连锁分子标记开发及NC82、K326品种抗性改良"(110201301009,JY-09) （JY-02）
中国烟草总公司科技重点项目"烤烟抗主要病毒病(TMV、CMV、PVY)基础材料创制及育种利用"(110201402006) （TMV、CMV、PVY）
中国农业科学院基本科研业务费专项"分子标记在烤烟品种DUS测试中的应用研究"(1610232016014) （1610232016014）
