中国烟草学报2018，Vol.24Issue(3)：82-87,6.DOI:10.16472/j.chinatobacco.2017.328
烟草灰霉病菌嘧菌酯抗药性突变菌株的诱导及其生物学习性分析
Induction and biological characters of laboratory mutants of Botrytis cinerea resistant to azoxystrobin
摘要
Abstract
The aim of current study was to investigate possible resistance risk of B.cinerea to azoxystrobin.[Methods] The azoxystrobin-resistant isolates were induced by UV mutation.Fungicide sensitivity and biological characters of all isolates were analyzed.Results showed that Azoxystrobin presented high inhibition against conidial germination of B.cinerea,with EC50value of 2.28 mg/L.Total five resistant isolates at different resistant levels were obtained by the UV mutation,and the range of resistant ratio was from 1.69 to 10.41.Biological character studies showed that all resistant isolates had stable sensitivity to the fungicide azoxystrobin after 11 continuous generations.The mycelial growth ratio of all resistant isolate was higher or lower than that of original isolate of B.cinerea.The conidia production and germination of all resistant isolates was lower than that of original isolate.The sclerotium production of resistant isolates was the same or lower than that of original isolate.All resistant isolates had pathogenicity on tobacco detached leaf,and some showed much stronger pathogenicity.[Conclusion] Azoxystrobin showed high activity against conidial germination of B.cinerea.If tobacco gray mold was controlled with azoxystrobin in the future,B.cinerea from tobacco had the fungicide resistant risk to azoxystrobin.关键词
烟草灰霉病/灰葡萄孢/抗药性/嘧菌酯Key words
tobacco gray mold/Botrytis cinerea/resistance/azoxystrobin引用本文复制引用
汪汉成,周浩,张之矾,陈兴江,蔡刘体,余知和..烟草灰霉病菌嘧菌酯抗药性突变菌株的诱导及其生物学习性分析[J].中国烟草学报,2018,24(3):82-87,6.基金项目
国家自然科学基金(31501679) （31501679）
贵州省科技厅优秀青年人才培养计划(黔科合平台人才[2017]5619) （黔科合平台人才[2017]5619）
中国烟草总公司贵州省烟草公司科技项目(201714) （201714）
中国博士后科学基金资助项目(2017M610585) （2017M610585）
