中国药房2018，Vol.29Issue(3)：330-333,4.DOI:10.6039/j.issn.1001-0408.2018.03.10
金钗石斛叶中总黄酮的提取分离及体外抗阿尔茨海默病活性研
Separation and Extraction of Total Flavonoids from Dendrobium nobile Leaves and Study on Its Anti-Alzheimer's Disease Activity in vitro
摘要
Abstract
OBJECTIVE: To separate and isolate total flavonoids from Dendrobium nobile leaves, and to investigate its anti-Alzheimer's disease (AD) activity in vitro. METHODS: Total flavonoids were obtained by ultrasonic extraction method and extracted by chloroform, ethyl acetate and butyl alcohol after the obtained extract was dispersed with water. Qualitative analysis was carried out with color reaction and TLC. The content of total flavonoids in extracts was analyzed quantitatively by Aluminum nitrate-sodium nitrite method. Antioxidant activity of extract was investigated by DPPH radical scavenging assay; the inhibitory effect of each extract on Aβ42 protein aggregation was investigated by Thioflavin T assay. Metals (Cu2+, Fe3+, Zn2+) chelating property was studied by UV-vis spectrum scanning to investigate the anti-AD activity in vitro. RESULTS: The flavonoids were found in ethyl acetate, butyl alcohol and aqueous extracts, and their flavonoids contents were 0. 03, 0. 12, 0. 05 mg/mL, respectively. IC50 of three extracts to DPPH free radicals were 0. 021, 0. 011, 0. 013 mg/mL. Inhibitory rates of them to Aβ42 protein aggregation were 43. 77％, 52. 28％, 38. 42％, respectively. Three extracts exerted metal chelating ability which was best in Cu2+. CONCLUSIONS: The total flavonoids from D. nobile leaves have good antioxidant activities, Aβ42 aggregation inhibitory activities and metal chelating activity, show certain anti-AD activity in vitro especially in butyl alcohol extract.关键词
金钗石斛叶/黄酮成分/阿尔茨海默病/抗氧化/Aβ42蛋白Key words
Dendrobium nobile leaves/Flavonoids/Alzheimer's disease/Antioxidant/Aβ42 protein分类
医药卫生引用本文复制引用
李艳萍,李海燕,纪晓婉,岑志芳,颜金武,吴剑峰..金钗石斛叶中总黄酮的提取分离及体外抗阿尔茨海默病活性研[J].中国药房,2018,29(3):330-333,4.基金项目
国家自然科学基金资助项目(No. 21502056) （No. 21502056）
广东省普通高校青年创新人才项目(No. 2014KQNCX182) （No. 2014KQNCX182）
