中国药房2018，Vol.29Issue(3)：361-364,4.DOI:10.6039/j.issn.1001-0408.2018.03.17
正交试验-多指标归一化法优化凤丹皮水提工艺
Optimization of Water Extraction Technology of Feng-Moutan Cortex by Orthogonal Test-Multiple-index Normalization Method
摘要
Abstract
OBJECTIVE: To optimize water extraction technology of Feng-Moutan Cortex. METHODS: L9 (34) orthogonal design was used to optimize soaking time, solid-liquid ratio, extraction time and extraction times in water extraction technology of Feng-Moutan Cortex using comprehensive scores for the peak area percentage and extract yield of 8 ingredients in Feng-Moutan Cortex as gallic acid, paeoniflorin, catechin, paeoniflorin, benzoic acid, benzoyloxypaeoniflorin, benzoylpaeoniflorin and paeonol after normalization as indexes. Verification test was conducted. RESULTS: The optimal water extraction technology was that 20-fold water, soaking for 1 h, extracting twice, 2 h each time. Results of verification test showed that comprehensive scores of 3 times of tests were 65. 98, 68. 85 and 69. 25, respectively. RSDs of each index were lower than 5％ (n=3). CONCLUSIONS: Established comprehensive scoring method can reflect the relative contents of effective components in Feng-Moutan Cortex comprehen- sively, so that optimized extraction technology is reasonable and feasible.关键词
凤丹皮/多指标/归一化法/正交试验/提取工艺/优化Key words
Feng-Moutan Cortex/Multiple-index/Normalization method/Orthogonal test/Extraction technology/Optimization分类
医药卫生引用本文复制引用
王培,赵宏苏,吴德玲,许凤清,张伟,龙全江,方成武..正交试验-多指标归一化法优化凤丹皮水提工艺[J].中国药房,2018,29(3):361-364,4.基金项目
安徽省2015年公益性技术应用研究联动计划(No. 15011d04001) （No. 15011d04001）
安徽高校自然科学研究项目(No. KJ2016A854) （No. KJ2016A854）
安徽道地中药材品质提升协同创新中心项目(No. 2014XTCX005) （No. 2014XTCX005）
