中国药房2018，Vol.29Issue(4)：455-457,3.DOI:10.6039/j.issn.1001-0408.2018.04.06
RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量
Simultaneous Determination of Shikonin, Acetylshikonin and β, β-dimethylacrylshikonin in Arnebia euchroma by RP-HPLC
摘要
Abstract
OBJECTIVE: To establish a method for the simultaneous determination of shikonin, acetylshikonin and β, β-dimethylacrylshikonin in Arnebia euchroma. METHODS: RP-HPLC method was adopted. The determination was performed on Kromasil 100-5 C18 column with mobile phase consisted of acetonitrile-0. 1％ formic acid solution (80: 20, V/V) at the flow rate of 1. 0 mL/min. The detection wavelength was set at 516 nm, column temperature was 25 ℃, and sample size was 10 μL. RESULTS: The linear ranges of shikonin, acetylshikonin and β, β-dimethylacrylshikonin were 0. 404-10. 100 μg/mL(r=0. 999 8), 5. 350-107. 000 μg/mL(r=0. 999 6), 2. 035-40. 700 μg/mL(r=0. 999 8), respectively. The limit of quantitation was 0. 40, 2. 91, 1. 34 μg/mL, and the limit of detection was 0. 12, 0. 87, 0. 40 μg/mL. RSDs of precision, stability and reproducibility tests were all lower than 2. 0％ (n=6). The recovery rate were 99. 12％-104. 18％ (RSD=1. 85％, n=6), 96. 51％-100. 21％ (RSD=1. 43％, n=6), 98. 11％-102. 51％ (RSD=1. 42％, n=6), respectively. CONCLUSIONS: The method is simple, precise, stable and reproducible. It can be used for simultaneous determination of shikonin, acetylshikonin and β, β-dimethylacrylshikonin in A. euchroma.关键词
紫草/紫草素/乙酰紫草素/β, β-二甲基丙烯酰紫草素/反相高效液相色谱法/含量Key words
Arnebia euchroma/Shikonin/Acetylshikonin/β/β-dimethylacrylshikonin/RP-HPLC/Content分类
医药卫生引用本文复制引用
孙健文,温宪春,蔡德富,王玥,吴永会..RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量[J].中国药房,2018,29(4):455-457,3.基金项目
国家自然科学基金资助项目(No. 81650021) （No. 81650021）
黑龙江省教育厅基本科研项目(No. 2016-KYYWF-0870) （No. 2016-KYYWF-0870）
