中国药房2018，Vol.29Issue(4)：461-465,5.DOI:10.6039/j.issn.1001-0408.2018.04.08
枳壳药材挥发油的GC-MS指纹图谱研究
Study on GC-MS Fingerprint of Volatile Oil from Citrus aurantium
摘要
Abstract
OBJECTIVE: To establish GC-MS fingerprint of volatile oil from Citrus aurantium. METHODS: GC-MS method was adopted. The determination was performed on RTX-5MS capillary column with injector temperature of 250 ℃, high pure helium as carrier gas(≥99. 999％), flow rate of 1. 0 mL/min, split ratio of 10:1,and sample size of 1 μL (temperature programming). Mass spectrum condition included electron bombardment ion source, ion source temperature of 230 ℃, detector temperature of 250 ℃, 3 min solvent delay, scanning range of m/z 35-550. GC-MS chromatograms of 21 batches of volatile oil samples were determined using Laurene as reference. The similarity of them was evaluated by using TCM Chromatographic Fingerprint Similarity Evaluation System (2004 A edition), and common peak was determined. The components of common peak were determined by LC Solution 2 mass database (NIST05. LIB and NIST05s. LIB). Relative content of common peak was determined with area normalization. RESULTS; There were 20 common peaks in GC-MS chromatograms of 21 batches of volatile oil samples, and the similarity was higher than 0. 90. After validation, GC-MS chromatograms of 21 batches of volatile oil samples were in good agreement with control fingerprint. The main constituents of the volatile oil of C. aurantium were Limonene, Terpinene, Laurene and D-Cadinene. CONCLUSIONS: Established fingerprint can provide reference for identification and quality evaluation of volatile oil of C. aurantium.关键词
枳壳/挥发油/气相色谱-质谱指纹图谱/道地药材Key words
Citrus aurantium/Volatile oil/GC-MS fingerprint/Genuine regional drug分类
医药卫生引用本文复制引用
刘清茹,谭伟民,文诗泳,肖幸华,陈婷,郭英,曾晓艳,刘塔斯..枳壳药材挥发油的GC-MS指纹图谱研究[J].中国药房,2018,29(4):461-465,5.基金项目
湖南省科技计划滚动项目(No. 2015skl001-1) （No. 2015skl001-1）
湖南省高校"2011协同创新中心"项目(No. 湘财教指〔2015〕69号) （No. 湘财教指〔2015〕69号）
