中国药房2018，Vol.29Issue(4)：470-473,4.DOI:10.6039/j.issn.1001-0408.2018.04.10
十大功劳叶药材的质量标准提高研究
Study on the Improvement of Quality Standard for Folium Mahoniae
摘要
Abstract
OBJECTIVE: To improve the quality standard of Folium Mahoniae. METHODS: TLC was used for qualitative identification. The contents of moisture, ash and ethanol extract were determined. The content of berberine hydrochloride was determined by HPLC. The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitrile-0. 05 mol/L monopotassium phosphate solution (25: 75, V/V) at the flow rate of 1. 0 mL/min. The detection wavelength was 264 nm, column temperature was 30 ℃, and sample size was 10 μL. RESULTS: TLC spots were clear and well-separated. The contents of moisture, total ash, acid-insoluble ash and ethanol extract were 3. 92％-7. 03％, 3. 65％-6. 95％, 0. 05％-1. 03％ and 10. 87％-33. 14％, respectively. The linear range of berberine hydrochloride was 0. 183-0. 915 μg(r=0. 999 9); quantitation limit and detection limit was 0. 143, 0. 095 μg, respectively. RSDs of precision, stability and reproducibility tests were lower than 2. 0％ (n=6); recovery was 95. 21％ -103. 10％ (RSD = 2. 95％, n=6). CONCLUSIONS: The content of moisture, total ash and acid-insoluble ash of medicinal materials is not exceed 8. 0％, 6. 0％ and 0. 4％, respectively. The content of ethanol extract and berberine hydrochloride is not less than 16. 0％ and 1. 0％, respectively. Established standard can be used for quality control of Folium Mahoniae.关键词
十大功劳叶/质量标准/高效液相色谱法/薄层色谱法Key words
Folium Mahoniae/Quality standard/HPLC/TLC分类
医药卫生引用本文复制引用
杨昌贵,肖承鸿,江维克,周涛,胡红莲..十大功劳叶药材的质量标准提高研究[J].中国药房,2018,29(4):470-473,4.基金项目
贵州省中药材、民族药材质量标准研究课题(No. 2015) （No. 2015）
贵州省研究生工作站建设项目(No. 黔教研合JYSZ字〔2014〕016) （No. 黔教研合JYSZ字〔2014〕016）
贵阳市科技计划项目(No. 筑科合同〔20151001〕药01号) （No. 筑科合同〔20151001〕药01号）
