中国药房2018，Vol.29Issue(4)：474-477,4.DOI:10.6039/j.issn.1001-0408.2018.04.11
小儿柴桂退热颗粒的UPLC指纹图谱及聚类、主成分分析
UPLC Fingerprint, Cluster and Principal Component Analysis of Xiao'er Chaigui Tuire Granules
林源 1陈敏1
作者信息
- 1. 澳门科技大学中医药学院, 澳门 999078
- 折叠
摘要
Abstract
OBJECTIVE: To establish UPLC fingerprint of Xiao'er chaigui tuire granules, and to conduct cluster analysis (CA) and principal component analysis (PCA). METHODS: UPLC method was adopted. The determination was performed on Waters Acquity UPLC BEH Ci8 column with mobile phase consisted of methanol-0. 05％ formic acid solution (gradient elution) at the flow rate of 0. 5 mL/min. The detection wavelength was 260 nm, the column temperature was 40 ℃, and sample size was 0. 5 μL. Using puerarin as reference, UPLC fingerprints of 14 batches of Xiao'er chaigui tuire granules were determined. The similarity of 14 batches of samples was evaluated by using TCM Chromatographic Fingerprint Similarity Evaluation System (2012 edition), and common peak was identified. CA and PCA were conducted by using SPSS 19. 0 software. RESULTS: There were 15 common peaks in UPLC chromatograms of 14 batches of samples. The similarity of UPLC fingerprints of 14 batches of samples was higher than 0. 90, and UPLC fingerprint was in good agreement with control fingerprint. The 14 batches of samples could be clustered into 2 categories. CONCLUSIONS: Established fingerprint can provide reference for quality evaluation of Xiao'er chaigui tuire granules.关键词
小儿柴桂退热颗粒/超高效液相色谱/指纹图谱/聚类分析/主成分分析/质量控制Key words
Xiao'er chaigui tuire granules/UPLC/Fingerprint/Cluster analysis/Principal component analysis/Quality control分类
医药卫生引用本文复制引用
林源,陈敏..小儿柴桂退热颗粒的UPLC指纹图谱及聚类、主成分分析[J].中国药房,2018,29(4):474-477,4.
