中国药房2018，Vol.29Issue(5)：615-620,6.DOI:10.6039/j.issn.1001-0408.2018.05.10
复方莪术油脂质体的制备及质量评价
Preparation and Quality Evaluation of Zedoary Turmeric Oil Compound Liposomes
摘要
Abstract
OBJECTIVE: To prepare zedoary turmeric oil compound liposomes (ZTOC-LPS) and evaluate its quality.METHODS: The preparation method of liposome, the addition amount of soybean phosphatidylcholine (SPC), ratio of SPC to cholesterol (CH) in lipid, drug-lipid ratio of zedoary turmeric oil (ZTO), drug-lipid ratio of tretinoin in formulation, and water bath temperature were screened using encapsulation efficiency and drug-loading amount of ZTO (represented by germacrone) and tretinoin as investigation indexes. Quality evaluation and primary stability investigation were conducted for liposomes prepared by optimal preparation technology. RESULTS: The optimal preparation method was ethanol injection method; The optimal preparation technology were SPC 4 mg in 1 mL lipid, the mass ratio of SPC to CH 3:1, the ratio of ZTO to lipid 1:9, the ratio of tretinoin to lipid 1:70, water bath temperature of 55 ℃. Encapsulation efficiencies of ZTO and tretinoin were (64. 63 ± 1. 00)％ and (90. 33 土 0. 72)％ in 3 batches of ZTOC-LPS, respectively. Drug-loading amount of ZTO and tretinoin were (9. 09 ± 0. 14)％ and (1. 43 ± 0. 02)％, respectively. Particle size was (257. 41 ± 7. 58) nm, Zeta potential was (-38. 77 ± 0. 81) mV,PDI was 0. 10 ± 0. 04; the results of centrifugal acceleration test showed that the liposomes had good physical stability. No obvious change was found in each investigation index of ZTOC-LPS that stored at (4 ± 2) ℃ for 1 month. CONCLUSIONS: Established preparation technology is simple and feasible, the quality of the prepared ZTOC-LPS conforms to the requirements, and it can provide reference for the following research of ZTOC-LPS.关键词
莪术油/维A酸/脂质体/包封率/载药量/制备工艺/质量评价Key words
Zedoary turmeric oil/Tretinoin/Liposomes/Encapsulation efficiency/Drug-loading amount/Preparation technology/Quality evaluation分类
医药卫生引用本文复制引用
陈积,赵小倩,胡姗姗,马彦巧,刘燕,谭丹枫,陈泽慧,田应彪..复方莪术油脂质体的制备及质量评价[J].中国药房,2018,29(5):615-620,6.基金项目
贵州省科技计划子课题(No.黔科合SY字〔2015〕3028-7) （No.黔科合SY字〔2015〕3028-7）
贵州省教育厅自然科学研究项目(No.黔教合KY字〔2012〕054号) （No.黔教合KY字〔2012〕054号）
贵州省中医药管理局中西药、民族医药科学技术研究课题(No.QZYY-2014-034) （No.QZYY-2014-034）
