中国药房2018，Vol.29Issue(5)：633-636,4.DOI:10.6039/j.issn.1001-0408.2018.05.14
HMGB1介导的炎症通路在雷公藤致大鼠肝损伤中的作用研究
Study on the Effects of HMGB1-mediated Inflammatory Pathway on Liver Injury Induced by Tripterygium wilfordii
董捷鸣 1章从恩 1于小红 1吴昊 1马致洁 1赵奎君1
作者信息
- 1. 首都医科大学附属北京友谊医院中药剂科, 北京 100050
- 折叠
摘要
Abstract
OBJECTIVE:To study the effects of high-mobility group box 1 protein(HMGB1) -mediated inflammatory pathway HMGB1-Toll like receptor 4 (TLR4) /nuclear transcription factor κ B(NF-κ B)on liver injury of rats induced by Tripterygium wilfordii,and to provide reference for clarify the mechanism of liver injury induced by T.wilfordii. METHODS:Totally 24 SD rats were randomly divided into blank group(normal saline,i. g. ),T. wilfordii group(16 g/kg by crude drug,i. g. )and neutralizer group(16 g/kg T. wilfordii crude drug i. g. after i. p injection of 100 mg/kg Ammonium glycyrrhizinate solution 3 h),with 8 rats in each group. All rats were treated for consecutive 3 weeks. The serum levels of AST and ALT in rats were detected every week. After the end of medication,the serum levels of HMGB1,IL-1β,IL-2 and TNF-α were detected by ELISA method;the protein expression of HMGB1,NF-κB p65 and TLR4 in liver tissue of rats were detected by Western blot assay. The pathological changes of liver tissue in rats were measured with HE staining method. RESULTS:After 3 weeks of treatment,the serum levels of AST, ALT,HMGB1,IL-1β,IL-2 and TNF-α in rats,the protein expression of HMGB1,NF-κB p65 and TLR4 in liver tissue of rats in T. wilfordii group were significantly higher than blank group and neutralizer group(P<0. 05 or P<0. 01). Hepatocyte edema was found around the central vein of the liver,and circular vacuoles were seen in some hepatic cytoplasm in T. wilfordii group;only varying size of vacuoles were found in a small number of cells in neutralizer group. CONCLUSIONS:T. wilfordii induced liver injury may be associated with the activation of HMGB1-TLR4/NF-κB inflammation pathway.关键词
雷公藤/肝损伤/高迁移率族蛋白1/Toll样受体4/核转录因子κB/大鼠Key words
Tripterygium wilfordii/Liver injury/High-mobility group box 1 protein/Toll like receptor 4/Nuclear transcription factor κB/Rats分类
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董捷鸣,章从恩,于小红,吴昊,马致洁,赵奎君..HMGB1介导的炎症通路在雷公藤致大鼠肝损伤中的作用研究[J].中国药房,2018,29(5):633-636,4.
