中国药房2018，Vol.29Issue(12)：1609-1612,4.DOI:10.6039/j.issn.1001-0408.2018.12.06
HPLC法同时测定桑菊感冒颗粒中5种有效成分的含量
Simultaneous Determination of 5 Effective Components in Sangju Ganmao Granules by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules. METHODS:HPLC method was adopted. The determination was performed on WondasilTM-C18 column with mobile phase consisted of acetonitrile-0.4% phosphoric acid solution(gradient elution)at the flow rate of 0.8 mL/min. The column temperature was set at 30 ℃,and the detection wavelength was set at 254 nm. The sample size was 5 μL. RESULTS:The linear range of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid were 0.014-0.560 μg (r＝0.999 2),0.022-0.880 μg(r＝0.999 6),0.010-0.400 μg(r＝0.999 9),0.016-0.640 μg(r＝0.999 9),0.011-0.440 μg(r＝0.999 1), respectively. The limits of quantitation were 0.752,0.647,0.483,0.372,0.242 mg/L;the limits of detection were 0.253,0.185, 0.157,0.162,0.098 mg/L. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The average recoveries were 97.4%-100.9%(RSD＝1.4%,n＝6),95.7%-99.0%(RSD＝1.1%,n＝6),95.1%-98.1%(RSD＝1.3%,n＝6),96.4%-99.8%(RSD＝1.4%,n＝6),97.8%-100.4%(RSD＝1.0%,n＝6),respectively. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,rutin,phillyrin,quercetin and glycyrrhizic acid in Sangju ganmao granules.关键词
桑菊感冒颗粒/高效液相色谱法/绿原酸/芦丁/连翘苷/槲皮素/甘草酸/含量测定Key words
Sangju gamao granules/HPLC/Chlorogenic acid/Rutin/Phillyrin/Quercetin/Glycyrrhizic acid/Content determination分类
医药卫生引用本文复制引用
崔佰吉,张宏梅,朱鹤云,冯波..HPLC法同时测定桑菊感冒颗粒中5种有效成分的含量[J].中国药房,2018,29(12):1609-1612,4.基金项目
国家自然科学基金资助项目(No.81703683) （No.81703683）
吉林省中医药科技项目(No.2017105) （No.2017105）
