中国药房2018，Vol.29Issue(12)：1640-1643,4.DOI:10.6039/j.issn.1001-0408.2018.12.13
竹节蓼药材的HPLC指纹图谱建立及聚类分析
Establishment of HPLC Fingerprint of Homalocladium platycladum and Cluster Analysis
摘要
Abstract
OBJECTIVE:To establish HPLC fingerprint of Homalocladium platycladum,and to carry on the cluster analysis. METHODS:HPLC method was adopted. The determination was performed on Ultimate C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid(gradient elution)at the flow rate of 0.5 mL/min. The detection wavelength was set at 360 nm, and column temperature was 28 ℃. The sample size was 10 μL. Using quercetin as reference substance,HPLC chromatograms of 13 batches of samples were determined. The similarity evaluation was carried out by using Similarity Evaluation System for TCM Chromatographic Fingerprint(2012 edition)to determine common peak. Cluster analysis were also conducted. RESULTS:A total of 12 common peaks were established in HPLC fingerprints of 13 batches of samples. The similarity was higher than 0.90. After validation,HPLC chromatograms of 13 batches of sample were in good agreement with control fingerprint. 13 batches of medicinal materials were clustered into 2 categories;S4,S6 and S10 were clustered into one category,and the rest were a category. CONCLUSIONS:Established fingerprint and cluster analysis results can provide reference for quality evaluation of H. platycladum and its exploitation and utilization.关键词
竹节蓼/高效液相色谱法/指纹图谱/聚类分析Key words
Homalocladium platycladum/HPLC/Fingerprint/Cluster analysis分类
医药卫生引用本文复制引用
黎理,曾祥燕,谢凤凤,蔡毅..竹节蓼药材的HPLC指纹图谱建立及聚类分析[J].中国药房,2018,29(12):1640-1643,4.基金项目
广西科学研究与技术开发计划项目(No.桂科重1598005-10) （No.桂科重1598005-10）
广西高校中青年教师基础能力提升项目(No.桂教科研〔2016〕3号-KY2016YB231) （No.桂教科研〔2016〕3号-KY2016YB231）
壮瑶药协同创新中心项目(No.桂教科研〔2013〕20号) （No.桂教科研〔2013〕20号）
广西壮瑶药重点实验室项目(No.桂科基字〔2014〕32号) （No.桂科基字〔2014〕32号）
广西教育厅重点学科项目(No.桂教科研〔2013〕16号) （No.桂教科研〔2013〕16号）
广西壮族自治区八桂学者项目(No.J13162) （No.J13162）
