中国医科大学学报2018，Vol.47Issue(1)：9-12,4.DOI:10.12007/j.issn.02584646.2018.01.003
结肠癌组织中miR-98的表达及作用机制
Expression and Action Mechanism of miR-98 in Colon Cancer Tissues
摘要
Abstract
Objective To investigate the expression and action mechanism of miR-98 in colon cancer tissues. Methods Tumor tissues and adjacent tissues were collected from 40 patients with colorectal cancer. The expression of miR-98 in tumor tissues and adjacent tissues was detected by real-time PCR. miR-98 was overexpressed or silenced in cells,and the effects on proliferation,cell cycle,and apoptosis were analyzed using MTT,flow cytometry,and Hoechst 33258 assays. Results Real-time PCR showed that the expression of miR-98 in tumor tissues was lower than that in adjacent tissues (P = 0.022). The survival rate of patients with lower miR-98 expression was shorter than that of patients with higher miR-98 expression. The MTT assay showed that miR-98 overexpression inhibited the proliferation of HCT116 cells. Hoechst 33258 staining showed that the overexpression of miR-98 could inhibit the cell cycle and promote the apoptosis of HCT116 cells. Conclusion miR-98 can inhibit the proliferation and promote the apoptosis of colon cancer cells. The expression of miR-98 is closely related to the survival of patients with colon cancer.关键词
miR-98/结肠癌/增殖/凋亡/生存期Key words
miR-98/colon cancer/proliferation/apoptosis/survival duration分类
医药卫生引用本文复制引用
王俊江,周天羽,张扬,刘明欣..结肠癌组织中miR-98的表达及作用机制[J].中国医科大学学报,2018,47(1):9-12,4.基金项目
国家自然科学基金(81202688) （81202688）
