中国医科大学学报2018，Vol.47Issue(1)：17-21,5.DOI:10.12007/j.issn.02584646.2018.01.005
载脂蛋白AⅠ通过诱导自噬抑制泡沫细胞脂质沉积和凋亡
Apolipoprotein AⅠInhibits Lipid Retention and Apoptosis in Foam Cells via Induction of Autophagy
摘要
Abstract
Objective To observe the effects of apolipoprotein AⅠ (apoAⅠ) on autophagy,lipid retention,and apoptosis in foam cells, and to explore the anti-atherosclerotic mechanism of apoAⅠ. Methods Macrophages derived from THP-1 cells were randomly divided into the control group,the 3-MA+apoAⅠ group,and the apoAⅠ group. Each group was administered oxidized low-density lipoprotein for 36 hours,then lipid droplets and autophagosomes were observed and cellular cholesterol content was quantified. LC3 and Beclin-1 expression was examined by western blot and the apoptotic ratio determined using flow cytometry. Results Compared with the results of the control group,apoAⅠ treatment inhibited lipid retention and apoptosis in foam cells,decreased cellular cholesterol content,and up-regulated the expression of LC3 and Beclin-1 (P < 0.01). Administration of 3-MA abrogated the effects of apoAⅠ (P < 0.05). Conclusion apoAⅠ inhibits lipid retention and apoptosis in foam cells by inducing autophagy.关键词
载脂蛋白AⅠ/自噬/脂质沉积/凋亡Key words
apolipoprotein AⅠ/autophagy/lipid retention/apoptosis分类
医药卫生引用本文复制引用
尹建国,张社兵,彭道泉..载脂蛋白AⅠ通过诱导自噬抑制泡沫细胞脂质沉积和凋亡[J].中国医科大学学报,2018,47(1):17-21,5.基金项目
国家自然科学基金(81370393) （81370393）
