中国医科大学学报2018，Vol.47Issue(2)：132-136,5.DOI:10.12007/j.issn.02584646.2018.02.009
通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响
Effects of Tongxinluo on High Glucose-induced Proliferation and Apoptosis of Cardiac Fibroblasts
摘要
Abstract
Objective This study investigated the effect of tongxinluo (TXL) on high glucose-induced proliferation and apoptosis of cardiac fibroblasts (CFs),to explore the possible mechanism by which TXL inhibits myocardial fibrosis. Methods Primary culture and subculture of neonatal SD rat CFs was carried out as follows. Immunofluorescence staining was performed to identify CFs. The CFs were divided into control group (cultured with low-glucose DMEM),model group (cultured with high-glucose DMEM),and TXL treatment groups (cultured with high-glucose DMEM+TXL at 20,80,and 320 μg/mL). The proliferation of CFs in each group was detected by MTT assay. The expression of collagen typesⅠand Ⅲ in each group was detected by ELISA. Western blotting was used to detect the expression of bax and bcl-2 in each group. Results CFs Proliferation and collagen typesⅠandⅢsecretion were higher in the model group than in the control group. The CFs proliferation and collagen content in the TXL treatment groups were significantly lower than those in the model group. bcl-2 expression was significantly increased and bax expression was decreased in the model group,compared with the corresponding values in the control group. In comparison with the model group,bcl-2 expression was downregulated and bax expression was upregulated in the TXL treatment groups. Conclusion TXL can reduce the CFs proliferation and inhibit their collagen secretion under high glucose conditions. TXL also induces the CFs apoptosis by reducing bcl-2 expression and increasing bax expression. Thus,TXL can play a role in the treatment of myocardial fibrosis.关键词
心肌成纤维细胞/高糖/通心络/增殖/凋亡Key words
cardiac fibroblasts/highglucose/tongxinluo/proliferation/apoptosis分类
医药卫生引用本文复制引用
于婷,王小梅..通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响[J].中国医科大学学报,2018,47(2):132-136,5.基金项目
辽宁省科学技术计划(2009225010-42) （2009225010-42）
