中国医科大学学报2018，Vol.47Issue(2)：157-161,5.DOI:10.12007/j.issn.02584646.2018.02.014
小鼠肝硬化—肝癌上皮间质转化的研究
Study of the Epithelial Mesenchymal Transition between Liver Cirrhosis and Liver Cancer in Mice
摘要
Abstract
Objective To establish animal models facilitating observation of the process of epithelial-mesenchymal transition between liver cirrhosis and liver cancer. Methods Liver cirrhosis and liver cancer models were established in mouse and subsequently assessed using HE and immunohistochemical staining. Results The success rate of the liver cirrhosis model was 100％ and the fatality rate was 30％. The success rate of the liver cancer model was also 100％ and the fatality rate was 40％. In the liver cirrhosis and liver cancer models,the positive expression rates were,respectively,46. 7％ and 7. 1％ for E-cadherin,40. 0％ and 7. 1％ for β3-integrin,13. 6％ and 40. 0％ for vimentin,and 20. 0％ and 57. 1％ for fibronectin. Conclusion In mouse models of liver cirrhosis and liver cancer,low expression levels of E-cadherin and β3-integrin in addition to high expression levels of fibronectin and vimentin are related to the epithelial-mesenchymal transition between liver cancer and cirrhosis,and may be important indicators for clinical detection.关键词
肝硬化模型/肝癌模型/E-钙黏蛋白/β3整联蛋白/波形蛋白/纤维连接蛋白/上皮间质转化Key words
liver cirrhosis model/liver cancer model/E-cadherin/β3-integrin/vimentin/fibronectin/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
孙洪利..小鼠肝硬化—肝癌上皮间质转化的研究[J].中国医科大学学报,2018,47(2):157-161,5.基金项目
沈阳市科学技术计划(F14-158-9-48) （F14-158-9-48）
