中国医科大学学报2018，Vol.47Issue(3)：193-197,5.DOI:10.12007/j.issn.0258-4646.2018.03.001
DMT1在APP/PS1转基因小鼠小脑皮质中表达上调
Elevation of Divalent Metal Transporter 1 Protein in the Cerebellar Cortex of the APP/PS1 Transgenic Mouse
摘要
Abstract
Objective To investigate the distribution of divalent metal transporter 1 (DMT1) in the cerebellum of APP/PS1 transgenic mouse. Methods Immunohistochemistry, immunofluorescence, and confocal laser scanning microscopy were used to analyze the relationship between DMTl and amyloid beta (Aβ) and their distribution in senile plaques. Western blotting was used to analyze DMT1 protein level in the APP/PS1 transgenic mouse cerebellum. Results DMTl and Aβ were mainly located in the amyloid plaques, which were predominately located in the molecular layer of the cerebellar cortex of the transgenic mouse. Only a few plaques could be seen in the Purkinje cell layer and granular layer. Confocal laser microscopy revealed the DMTl and Aβ were co-localized in senile plaques. Conclusion The abundant expression of DMTl protein suggests that DMTl and the divalent metal ions that it transports might be involved in the formation of Aβ senile plaques and other pathological processes in the cerebellum in Alzheimer' s disease.关键词
二价金属离子转运体1/β-淀粉样蛋白/APP/PS1转基因小鼠/小脑/阿尔茨海默病Key words
divalent metal transporter 1/amyloid beta/APP/PS1 transgenic mouse/cerebellum/Alzheimer ' s disease分类
医药卫生引用本文复制引用
王思亓,李欣潞,林庚,王卓,程晓凤,刘彤彤,郑玮..DMT1在APP/PS1转基因小鼠小脑皮质中表达上调[J].中国医科大学学报,2018,47(3):193-197,5.基金项目
国家自然科学基金(81471112) （81471112）
辽宁省自然科学基金(2014021033) （2014021033）
