中国医科大学学报2018，Vol.47Issue(3)：202-205,211,5.DOI:10.12007/j.issn.0258-4646.2018.03.003
microRNA-1183通过抑的表达促进胃癌细胞的增殖及转移
microRNA-1183 Promotes the Proliferation and Metastasis by Suppressing the Expression of CBL-B in Gastric Cancer Cells
摘要
Abstract
Objective To investigate the effect of microRNA-1183 on proliferation and metastasis on gastric cancer cells and to explore the role of microRNA-1183 and CBL-B signaling pathways in this process. Methods MGC803 cells were transfected with a microRNA-1183 mimic. Real-time PCR detected the expression of microRNA-1183 in gastric cancer cell line MGC803. MTT detected the proliferative effect of microRNA-1183 on MGC803 gastric cancer cells. A Transwell assay detected the effect of microRNA-1183 on the metastasis of MGC803 gastric cancer cells. A dual luciferase reporter assay detected the binding ability between microRNA-1183 and CBL-B. The expression of the protein was tested by Western blotting. Results MTT assay results showed that microRNA-1183 promoted the proliferation of MGC803 cells. Transwell assay results revealed that microRNA-1183 promoted the metastasis of MGC803 cells. The results of BLAST contrast analysis show that CBL-B is one of the target genes of microRNA-1183. Western blotting analysis showed that the mimic microRNA-1183 inhibited the expression of CBL-B. A dual luciferase reporter assay showed that CBL-B was the target gene of microRNA-1183. A CBL-B knockdown promoted the proliferation and metastasis of MGC803 cells. microRNA-1183 promoted the proliferation and metastasis of MGC803 cells by inhibiting the expression of CBL-B. Conclusion microRNA-1183 can inhibit the proliferation and metastasis of gastric cancer cell lines by inhibiting the expression of CBL-B.关键词
胃癌/microRNA-1183/CBL-B/增殖/转移Key words
gastric cancer cells/microRNA-1183/CBL-B/proliferation/metastasis分类
医药卫生引用本文复制引用
范一博,车晓芳,赵欢,胡雪君,曲秀娟,刘云鹏..microRNA-1183通过抑的表达促进胃癌细胞的增殖及转移[J].中国医科大学学报,2018,47(3):202-205,211,5.基金项目
国家自然科学基金(81602098) （81602098）
辽宁省科技厅计划(2014226033) （2014226033）
辽宁省科技厅中央引导地方科技发展专项资金计划(2016007010) （2016007010）
