中国医科大学学报2018，Vol.47Issue(3)：217-221,5.DOI:10.12007/j.issn.0258-4646.2018.03.006
β-淀粉样蛋白诱导的阿尔茨海默病大鼠模型中BDNF与TRPC3的关系
Relationship between BDNF and TRPC3 in a Rat Model of Alzheimer's Disease Induced by β-amyloid Protein
摘要
Abstract
Objective To investigate the expression and relationship of canonical transient receptor potential channel-3 (TRPC3) and brain-derived neurotrophic factor (BDNF) in the hippocampus of a rat model of Alzheimer's disease (AD). Methods SD rats were randomly divided into PBS, AD, and AD+BDNF experimental groups. AD models were generated by intracerebroventricular injection ofβ-amyloid protein (Aβ1-42). BDNF was injected via the lateral ventricle catheter after 14 days. The Morris water maze test was used to assess the spatial learning and memory ability of the rats. The expression of TRPC3 and BDNF mRNA and protein in the hippocampus were detected by RT-PCR and Western blotting, respectively. Results The Morris water maze test showed that the escape latencies of the fifth day in the AD group were longer than those in the PBS group (P ＜ 0. 05). The escape latencies in the AD+BDNF group were shorter than those in the AD group (P ＜ 0. 05). RT-PCR and Western blotting results showed that the expression of both TRPC3 and BDNF were reduced in the AD group compared with the PBS group (P ＜ 0. 05). TRPC3 expression was increased in the AD+BDNF group compared with the AD group (P ＜ 0. 05). Conclusion The expression of BDNF and TRPC3 is decreased in the hippocampus of AD rats. An exogenous BDNF injection appears to improve the spatial learning and memory of AD rats that are impaired by a Aβ1-42 injection, possibly via TRPC3 upregulation, and may play a protective role in neurons.关键词
经典瞬时受体电位通道蛋白3/阿尔茨海默病/β-淀粉样蛋白/脑源性神经营养因子Key words
canonical transient receptor potential channel-3/Alzheimer ' s disease/β-amyloid protein/brain-derived neurotrophic factor分类
医药卫生引用本文复制引用
张利华,张丽艳,张小康,徐超龙,周义..β-淀粉样蛋白诱导的阿尔茨海默病大鼠模型中BDNF与TRPC3的关系[J].中国医科大学学报,2018,47(3):217-221,5.基金项目
辽宁省自然科学基金(20170540874) （20170540874）
沈阳医学院科学研究基金(20171008) （20171008）
辽宁省大学生创新创业训练计划(201610164002) （201610164002）
