中国医科大学学报2018，Vol.47Issue(3)：226-230,5.DOI:10.12007/j.issn.0258-4646.2018.03.008
白三烯受体拮抗剂通过Wnt/β-catenin信号通路影响哮喘小鼠气道重塑机制的研究
Effects of a Leukotriene Receptor Antagonist on Airway Remodeling in Asthmatic Mice via the Wnt/β -catenin Signaling Pathway
摘要
Abstract
Objective To investigate the expression of Wnt7b, β -catenin, and c-Myc in asthmatic mice and the intervention of the leukotriene receptor antagonist montelukast on airway remodeling. Methods The asthma model was established by ovalbumin (OVA) induction. HE staining was used to observe the pathological changes in lung tissue. Serum OVA-sIgE levels were determined by ELISA. The level of Wnt7b, β -catenin, and c-Myc protein and mRNA in the lung tissue of mice was analyzed by Western blotting and real-time PCR. The basement membrane perimeter (PBM), wall area of bronchial tube (WAt), wall area of bronchial smooth muscle (WAm), and the number of smooth muscle cells were measured using medical image analysis software and standardized based on the PBM. Results The amount of OVA-slgE in the asthma group was significantly higher than in the control and montelukast groups (P ＜ 0. 05). Western blotting and real-time PCR showed that the expression of Wnt7b, β -catenin, and c-Myc in the asthma group was higher than the expression in the control and montelukast groups (P ＜ 0. 05). Image analysis showed that the WAt/PBM and WAm/PBM ratios in the montelukast group were significantly lower than those in the asthma group (P ＜ 0. 05). Conclusion The Wnt/ β -catenin signaling pathway may be an important factor in the pathogenesis of asthma; montelukast may attenuate airway remodeling in asthmatic patients by decreasing the expression of Wnt7b, β -catenin, and c-Myc.关键词
Wnt7b/β-catenin/c-Myc/哮喘/气道重塑/白三烯受体拮抗剂Key words
Wnt7b/β -catenin/c-Myc/asthma/airway remodeling/leukotriene receptor antagonists分类
医药卫生引用本文复制引用
蒋昱,黄传君,李泽,张才擎..白三烯受体拮抗剂通过Wnt/β-catenin信号通路影响哮喘小鼠气道重塑机制的研究[J].中国医科大学学报,2018,47(3):226-230,5.基金项目
山东省自然科学基金(ZR2014HL003) （ZR2014HL003）
