中国医科大学学报2018，Vol.47Issue(3)：247-250,259,5.DOI:10.12007/j.issn.0258-4646.2018.03.013
抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响
Effect of Low Rabll Expression on the Proliferation and Invasion of Human Bladder Cancer Cells
摘要
Abstract
Objective To investigate the effect of inhibition of Rabll expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rabll siRNA, and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rabll expression on cell proliferation, cell cycle progression, and cell invasion was analyzed by performing CCK8, cell cycle detection, and Matrigel invasion assays, respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9 (MMP9) was determined by performing Western blotting and RT-PCR. Results Inhibition of Rabl 1 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rabl 1 functions as a tumor protein and is involved in the progression of bladder cancer.关键词
Rabll/膀胱尿路上皮癌/增殖/侵袭Key words
Rabll/bladder urothelial carcinoma/proliferation/invasion分类
医药卫生引用本文复制引用
宫雪,于柳,祝兴旺,刘嘉,刘屹立,王平..抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响[J].中国医科大学学报,2018,47(3):247-250,259,5.基金项目
辽宁省科学技术计划(2015225009) （2015225009）
