中国药理学通报2018，Vol.34Issue(7)：959-963,5.DOI:10.3969/j.issn.1001-1978.2018.07.014
白藜芦醇调节SphK1/AP-1信号通路抑制高糖诱导的肾小球系膜细胞增殖
Effects of resveratrol on cell proliferation in glomerular mesangial cells cultured under high glucose by inhibiting SphK1/AP-1 signal pathway
摘要
Abstract
Aim To observe the expression of FN and TGF-β1 in the glomerular mesangial cells induced by high-glucose after the intervention of resveratrol, and further discuss its influence on SphK1/AP-1 signaling pathway. Methods The rat glomerular mesangial cells induced by high glucose were used to observe the effects of resveratrol on cell proliferation after interven-tion. The survival vitality and proliferation of glomeru-lar mesangial cells were determined by MTT, and then FN, TGF-β1 and SphK1 protein expression were deter-mined by Western blot. Also, AP-1 activity was deter- mined by EMSA assay. Results Resveratrol could obviously inhibit the proliferation of high glucose-in-duced glomerular mesangial cells, lower SphK1 expres-sion, inhibit AP-1 activity and thus inhibit the expres-sion of FN, TGF-β1. Conclusions Resveratrol inhib-its the proliferation of high glucose-induced glomerular mesangial cells, which may be closely related to the in-hibition of SphK1/AP-1 signaling pathway.关键词
白藜芦醇/糖尿病肾病/细胞增殖/SphK1/AP-1信号通路/纤维连接蛋白/转化生长因子β1Key words
resveratrol/diabetic nephropathy/cell proliferation/SphK1/AP-1 signaling pathway/fibrone-ctin/transformation growth factor β1分类
医药卫生引用本文复制引用
邓艳辉,公文艳,李强,伍飞臻,段萍萍,黄河清..白藜芦醇调节SphK1/AP-1信号通路抑制高糖诱导的肾小球系膜细胞增殖[J].中国药理学通报,2018,34(7):959-963,5.基金项目
国家自然科学基金资助项目( No 81603355) （ No 81603355）
广东省中医药管理局项目(No 20161154) （No 20161154）
