中国药理学通报2018，Vol.34Issue(7)：1024-1029,6.DOI:10.3969/j.issn.1001-1978.2018.07.025
高内涵细胞成像系统在细胞增殖检测中的应用
Application of high-content cell imaging system in cell proliferation detection
摘要
Abstract
Aim To compare high-content cell imaging system and other methods in detecting cell proliferation, including the traditional thymidine ( 3H-TdR) incorporation method, methyl thiazolyl tetrazolium ( MTT ) method and cell counting kit-8 (CCK-8) method. Methods The fibroblast-like synovial cells (FLS) were used as the study object to observe the sensitivity and stability of FLS proliferation in different methods by using the usual proliferative stimulant tumor necrosis factor-α ( TNF-α) and the known proliferation inhibitor methotrexate at different concentrations. Results The 3H-TdR method and high-content cell imaging could detect a significant inhibitory effect of 1 nmol ·L-1 MTX on FLS cell proliferation, MTT assay and CCK-8 method could detect the significant inhibitory effect of 10 nmol· L-1 MTX on FLS cell proliferation. 3H-TdR method was found to have a large degree of discretization in the data set, with a standard deviation of 32.61% ~61.36% , and the MTT method was 11.9% ~ 17.8% , the CCK-8 method was 17.15% ~32.88% , and the high-content cell imaging system method was 12.66% ~26.54%. Conclusion The method of high-content cell imaging system is more accurate and stable for detecting cell proliferation.关键词
细胞增殖/高内涵细胞成像/MTT法/3H-TdR参入法/CCK-8法/成纤维样滑膜细胞/TNF-α/甲氨蝶呤Key words
cell proliferation/high-content cell imaging sys-tem/MTT/3 H-TdR participation method/CCK-8/fibroblast-like synoviocytes/TNF-α/methotrexate分类
医药卫生引用本文复制引用
吴莉,邰宇,胡珊珊,王瑞,张梅,陶娟,周伟杰,汪庆童,魏伟..高内涵细胞成像系统在细胞增殖检测中的应用[J].中国药理学通报,2018,34(7):1024-1029,6.基金项目
国家自然科学基金资助项目( No 81173075,81330081, 81202541) （ No 81173075,81330081, 81202541）
安徽高校自然科学研究项目( No KJ2017A 176) （ No KJ2017A 176）
安徽省高校优秀青年人才支持计划 ( No gxyqZD2017025) （ No gxyqZD2017025）
安徽医科大学青年拔尖人才支持计划 （）
安徽医科大学校级中青年学术技术带头人基金 （）
