中国油料作物学报2018，Vol.40Issue(3)：335-343,9.DOI:10.7505/j.issn.1007-9084.2018.03.004
花生苗期干旱处理后转录和代谢通路分析
Molecular analysis of formation of drought tolerance traits in peanut
摘要
Abstract
In order to elucidate the regulating basis of drought tolerance in peanut,the experiments of drought and re-water after drought were carried out on 10 different peanut accessions to select drought tolerant and sensi-tive lines,combined with the transcriptome to analysis the regulation mechanism of drought resistance of different peanut lines.Waliyar Tiga,which was collected from Africa,showed the best drought tolerance,followed by kQ044kangqing,Zhonghua 16 and Zaohuasheng.The physiological results showed that there were significant differ-ences in root/shoot ratio between drought-tolerant and drought-sensitive peanuts.Transcriptome analysis demon-strated that differential expressed genes between drought tolerant and susceptible peanut lines were obviously en-riched in oxidative phosphorylation,photosynthesis and secondary metabolites pathways;the genes expression of auxin signal pathway in drought-tolerant peanuts lines were significantly lower than that in the susceptible ones.The physiological and transcriptome data demonstrated that enlarged root system,enhanced energetic,secondary metabolism and the inhibition of growth were the four aspects contributing to drought stress tolerance in peanut.Both Zhonghua 16 and Waliyar Tiga have vigorous root and energetic metabolism,and the root shoot ratio was even larger in Zhonghua 16 than Waliyar Tiga,the reason for the conflict could be caused by the larger leaf area which resulted greater water loss.This study provided a theoretical basis for drought-tolerant peanuts breeding,and pro-vided a way to the further study drought tolerance regulation mechanism of peanut.关键词
花生/耐旱/转录组/根系Key words
peanut/drought tolerance/transcriptome/root system分类
农业科技引用本文复制引用
万丽云,廖伯寿,苏威,李蓓,雷永,晏立英,康彦平,淮东欣,陈玉宁,姜慧芳..花生苗期干旱处理后转录和代谢通路分析[J].中国油料作物学报,2018,40(3):335-343,9.基金项目
国家自然科学基金(31461143022) （31461143022）
国家花生产业技术体系(CARS-14) （CARS-14）
