中国中医药信息杂志2018，Vol.25Issue(7)：53-56,4.DOI:10.3969/j.issn.1005-5304.2018.07.013
肝豆灵对高铜负荷小鼠神经干细胞活性氧水平及核因子E2相关因子2表达的影响
Effects of Gandouling on ROS and Nrf2 of Neural Stem Cells of Mice Cultured in High Concentration Copper
摘要
Abstract
Objective To observe the effects of Gandouling on reactive oxygen species (ROS) level, the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA and protein of neural stem cells of the mice cultured in high concentration copper. Methods The model of neural stem cells of the mice was cultured in vitro with high concentration copper. The experimental rats were randomly divided into blank control group, model group, and Gandouling low-, medium-, and high-dose groups. Each medication group was given relevant concentration of Gandouling serum for gavage. The MTT was adopted to test proliferation level on neural stem cells; flow cytometer was used to examine the change of ROS level in cells; qPCR was used to measure the expression of Nrf2 mRNA;Western blot was used to measure the change of the level of protein Nrf2 in cells. Results Compared with the blank control group, the proliferation rate of neural stem cells was significantly decreased, ROS levels were significantly increased, and Nrf2 gene and protein expression was significantly decreased (P＜0.01). Compared with the model group, neural stem cells proliferation rate was significantly increased, ROS levels were significantly reduced, and Nrf2 gene and protein expression was significantly increased (P＜0.05, P＜0.01). Conclusion Gandouling can promote the proliferation of neural stem cells in mice by reducing ROS content in high copper-loaded mice and up-regulating Nrf2 expression.关键词
肝豆灵/高铜负荷/体外培养/神经干细胞/活性氧/核因子E2相关因子2/小鼠Key words
Gandouling/high copper load/cultured in vitro/neural stem cells/reactive oxygen species/nuclear factor erythroid 2-related factor 2/mice分类
医药卫生引用本文复制引用
董婷,杨文明,吴明彩,蒋怀周,黄鹏,匡春俊,张娟,韩辉..肝豆灵对高铜负荷小鼠神经干细胞活性氧水平及核因子E2相关因子2表达的影响[J].中国中医药信息杂志,2018,25(7):53-56,4.基金项目
国家自然科学基金面上项目(81774299) （81774299）
安徽省自然科学基金面上项目(1808085MH263) （1808085MH263）
