中国中医药信息杂志2018，Vol.25Issue(7)：71-74,4.DOI:10.3969/j.issn.1005-5304.2018.07.017
护肾(Ⅲ)号胶囊中三七皂苷R1和人参皂苷Rg1在大鼠体内药代动力学研究
Pharmacokinetic Study on Notoginsenoside R1 and Ginsenoside Rg1 in Hushen (Ⅲ) Capsules in Rats
摘要
Abstract
Objective To establish a UHPLC-MS/MS method for the determination of notoginsenoside R1 and ginsenoside Rg1 in rat plasma after oral administration of Hushen (Ⅲ) Capsules; To study its pharmacokinetics. Methods SD rats were given Hushen (Ⅲ) Capsules 2 g/kg and then the blood was collected at time point. Digoxin was used as internal standard. The drug concentrations of notoginsenoside R1 and ginsenoside Rg1 in plasma were determined by UHPLC-MS/MS. And the relative pharmacokinetic parameters were calculated by using DAS2.0 software. Results The linear relationship of notoginsenoside R1 and ginsenoside Rg1 was good (r≥0.997 5). The RSD of intra-day and inter-day precision were less than 13.1%. The results of accuracy, recovery and stability met the requirements for the biology sample analysis. For notoginsenoside R1, the pharmacokinetics parameters T1/2, Tmax, Cmaxin the plasma were (7.86±1.69)h, (4.00±1.04)h, (0.23±0.05)mg/L. For ginsenoside Rg1, the pharmacokinetics parameters T1/2, Tmax, Cmaxin plasma were (4.58±0.95)h, (6.00±0.00)h, (0.32±0.03)mg/L. Conclusion The established UHPLC-MS/MS method is sensitive, accurate and reliable, and is suitable for the pharmacokinetic study of Hushen (Ⅲ) Capsules.关键词
护肾(Ⅲ)号胶囊/超高液相色谱串联质谱/药代动力学Key words
Hushen (Ⅲ) Capsules/UHPLC-MS/MS/pharmacokinetics分类
医药卫生引用本文复制引用
钱伟,冯星月,陈晓峰,王建平,蒋志涛,余辉..护肾(Ⅲ)号胶囊中三七皂苷R1和人参皂苷Rg1在大鼠体内药代动力学研究[J].中国中医药信息杂志,2018,25(7):71-74,4.基金项目
全国中药特色技术传承人才培训项目(2015年) （2015年）
