中华骨质疏松和骨矿盐疾病杂志2018，Vol.11Issue(3)：267-273,7.DOI:10.3969/j.issn.1674-2591.2018.03.008
地塞米松对大鼠成骨细胞功能及蛋白激酶B磷酸化的影响
Effect of dexamethasone on osteoblast function and Akt phosphorylation in rats
摘要
Abstract
Objective To investigate the effects of different concentrations of dexamethasone (Dex) on osteoblast funtion and Akt phosphorylation in rats. Methods Osteoblasts were acquired by primary culturing from new born SD rats. Cells were divided into different groups as follows: control group (0 mol/L Dex), 10-8mol/L Dex group, 10-7mol/L Dex group, and 10-6mol/L Dex group, and were incubated for 24 hours. To further verify the effects of dexamethasone on prolif-eration and function of osteoblasts, cells were divided into control group ( 0 mol/L Dex, 0 mol/L SC79), SC79 group (0 mol/L Dex, 10 μmol/L SC79), Dex group (10-6mol/L Dex, 0 mol/L SC79), SC79+Dex group (10 μmol/L SC79, 10-6mol/L Dex), and were incubated for 24 hours. Cell counting Kit-8 assay was performed to evaluate the cell proliferation. Alkaline phosphatase (ALP) kit was used to detect the content of ALP in osteoblast supernatant. Western blot assay examined the expression of ALP, Akt and phosphorylated Akt (p-Akt) in each group. Results Compared with the control group, the osteoblasts proliferation in 10-7mol/L and 10-6mol/L Dex groups were decreased (A value: 0. 742±0. 022, 0. 598±0. 028, 0. 570±0. 025, P<0. 05), and 10-8mol/L dexamethasone had no obvious inhibitory effect on osteoblast proliferation (P>0. 05). The activity of ALP in all Dex groups except 10-8mol/L groups was significantly lower than control group (ALP ac-tivity value: 0. 316±0. 015, 0. 313±0. 013, 0. 351±0. 028, P<0. 05). Dexamethasone could down-regulate the expression of p-Akt and ALP, at the concentration of 10-8mol/L, 10-7mol/L and 10-6mol/L. The expressions of p-Akt were marked-ly reduced by 16. 9%, 34. 9%, 62. 5% (P<0. 05), and ALP protein expression was reduced by 16. 2%, 24. 8% and 69. 3%. There were no significant difference between 10-8mol/L Dex group and control group. Compared with the control group, 10 μmol/L SC79 could up-regulate the expression of p-Akt and ALP (P<0. 05). Compared to the Dex group, the proliferation rate and ALP activity of SC79+Dex group were markedly increased (P<0. 05), and the expression of p-Akt and ALP protein increased significantly. Conclusion High concentration of dexamethas one can inhibit the proliferation of osteoblasts and the expression of ALP, and its mechanism may be related to the down-regulation of Akt phosphorylation.关键词
地塞米松/骨质疏松/成骨细胞/蛋白激酶BKey words
dexamethasone/osteoporosis/osteoblast/Akt分类
医药卫生引用本文复制引用
潘吉铭,蔡劲薇,伍龙果,梁敏..地塞米松对大鼠成骨细胞功能及蛋白激酶B磷酸化的影响[J].中华骨质疏松和骨矿盐疾病杂志,2018,11(3):267-273,7.基金项目
国家自然科学基金 (81260142, 81760165) （81260142, 81760165）
