浙江医学2018，Vol.40Issue(9)：899-901,928,4.DOI:10.12056/j.issn.1006-2785.2018.40.9.2017-3148
双氢青蒿素对MRL/lpr SLE小鼠CD4+T细胞基因组DNA甲基化水平的影响研究
Effect of dihydroatermisinin on genome DNA hypermethylation in CD4+ T cells of MRL/lpr SLE mice in vitro
摘要
Abstract
Objective To investigate the effect of dihydroatermisinin on genome DNA hypermethylation in CD4+ T cells of MRL/lpr SLE mice in vitro. Methods CD4+ T cells were isolated from spleen of MRL/lpr SLE mice and were cultured with dihydroartemisinin. Cell toxicity of dihydroartemisinin was tested by CCK-8 kits. Global DNA methylation in CD4+ T cells was examined with high-performance liquid chromatography (HPLC) analysis. The expression of DNMT1 and Gadd45a mRNA and protein were detected with RT-PCR and Western-blot, respectively. Results The IC50 of dihydroartemisin on CD4+ T cells of MRL/lpr mice was 62μmol/L. The proliferation of CD4+ T cells was inhibited by dihydroartemisinin in a concentration-dependent manner. Global DNA methylation in CD4+ T cells was increased after dihydroartemisinin treatment in a concentration-dependent manner. The expression of DNMT1 mRNA and protein was increased, while the expression of Gadd45a was decreased after dihydroartemisinin treatment, the effects were related to concentration of dihydroartemisinin. Conclusion Dihydroartemisinin can increase the genome DNA methylation in CD4+ T cells and inhibit CD4+ T cell proliferation in MRL/lpr SLE mice, indicating that it may be used to treat systemic lupus erythematosus.关键词
双氢青蒿素/系统性红斑狼疮/DNA甲基化/DNMT1/Gadd45aKey words
Dihydroartemisinin/Systemic lupus erythematosus/DNA methylation/DNMT1/Gadd45a引用本文复制引用
陈红波,项晓骏,范军芬,郭小文,寿旗扬,马红珍,范永升..双氢青蒿素对MRL/lpr SLE小鼠CD4+T细胞基因组DNA甲基化水平的影响研究[J].浙江医学,2018,40(9):899-901,928,4.基金项目
国家自然科学基金项目(81673919、81202673) （81673919、81202673）
