浙江医学2018，Vol.40Issue(9)：906-909,918,后插2,6.DOI:10.12056/j.issn.1006-2785.2018.40.9.2017-3058
沉默β-catenin表达对胃癌MGC-803细胞生物学行为的影响与作用机制研究
Effect of Silencing β-catenin expression on biological behaviors of gastric cancer MGC-803 cells and related molecular mechanisms
摘要
Abstract
Objective To investigate the effect of silencing β -catenin gene expression on biological behaviors of gastric carcinoma cells and the related molecular mechanisms. Methods Human gastric cancer MGC-803 cells were infected with β -catenin-RNAi lentivirus (study group) and β -catenin-negative lentivirus (negative control group), respectively. The expression of β -catenin,Bax,Bcl-2, cyclin and MMP-7 proteins was detected by Western blotting. The apoptosis, cell cycle and migration of infected and non-infected (normal control group) MGC-803 cells were determined by flow cytometry and Transwell assay, respectively. Results The expression levels of β -catenin in study group was lower than that of the other two groups(P＜0.05). Cell apoptosis was increased(P＜0.05), cell cycle was blocked at G1/S phase(P＜0.05) and the migration ability was inhibited in MGC803 cells after infected with β -catenin-RNAi lentivirus (P＜0.05). The expression of Bcl-2, cyclin D1 and MMP-7 proteins was inhibited but the expression of Bax was increased in MGC cells after β -catenin expression was silenced. Conclusion The silence of β -catenin gene expression can lead to increasing cell apoptosis, inhibiting cell cycle and migration ability in gastric cancer MGC-803 cells, which are associated with the down-regulation of f Bax, Bcl-2, cyclin D1 expression and up-regulation of MMP-7 expression.关键词
胃癌/MGC-803细胞/β-catenin/生物学行为Key words
Gastric cancer/MGC-803 cell/β-catenin/Biological behavior引用本文复制引用
潘安萍,朱建伟,王忻妍..沉默β-catenin表达对胃癌MGC-803细胞生物学行为的影响与作用机制研究[J].浙江医学,2018,40(9):906-909,918,后插2,6.
