浙江医学2018，Vol.40Issue(10)：1027-1032,后插1,7.DOI:10.12056/j.issn.1006-2785.2018.40.10.2017-3008
体外模拟SAH状况下IL-6对大鼠蛛网膜细胞极化状态的影响
Effects of IL-6 on polarization of rat arachnoid cells in mimic subarachnoid hemorrhagic condition in vitro
摘要
Abstract
Objective To investigate the effect of interleukin-6 (IL-6) on the polarization state of rat arachnoid (Ar) cells in mimic subarachnoid hemorrhage (SAH) condition in vitro. Methods The mimic condition in vitro was induced by activation of thrombin on cultured rat arachnoid cells. Expression of CK8+18, Vimentin and CD68 in rat arachnoid cells was detected by immunocytochemistry (ICC). The changes of IL-12, IL-6 and IL-10 expression were detected by ELISA method after Ar cells were stimulated with GM-CSF and M-CSF. The mRNA expression of CD68, CK8+18, IL-12, IL-23 and Arg-I in stimulated Ar cells and non-activated Ar cells was detected by real-time fluorescence quantitative RT-PCR (qRT-PCR). Results The expressions of Ck8+18, Vimentin and CD68 in Ar cells and CD68 expression in macrophages were positive. The expression of IL-6 and IL-12p40 increased after GM-CSF stimulation, while the expression of IL-10 was not changed after M-CSF stimulation in Ar cells. Thrombin induced high Arg-I M2 polarization in Ar cells. When stimulated by the large dose IL-6, the CD68 was expressed early and shortly. The expression of IL-23 was increased in the SAH group at the 1d, 3d, 76 and 14d, while the expression of IL-23, IL-12p40 and Arg-I in the control group only increased 14d after stimulation. Conclusion The cultured Ar cells have the characteristics of Macrophages. Ar cells only can be induced to M1 polarization, not to M2 polarization under mimic SAH condition, the poor polarizability may be unfavorable for the elimination of hemolytic products in subarachnoid space resulting in SAH related complications. After IL-6 stimulation Ar cells show M1/M2 polarization, which provides new evidence for IL-6 as a prognostic factor of SAH.关键词
蛛网膜下腔出血/蛛网膜细胞/细胞极化/IL-6Key words
Subarachnoid hemorrhage/Arachnoid cells/Cell polarization/Interleukin-6引用本文复制引用
陈功,陈祥,朱丹丹,王自谱,张小洁,信照亮..体外模拟SAH状况下IL-6对大鼠蛛网膜细胞极化状态的影响[J].浙江医学,2018,40(10):1027-1032,后插1,7.基金项目
国家自然科学基金(81241044) （81241044）
浙江省公益技术研究社会发展项目(2013C33200) （2013C33200）
