浙江医学2018，Vol.40Issue(10)：1033-1036,1053,5.DOI:10.12056/j.issn.1006-2785.2018.40.10.2017-1016
系统性红斑狼疮患者B细胞中microRNA-155的表达及干预后效应
Expression of miRNA-155 in peripheral B cells of patients with systemic lupus erythematosus
摘要
Abstract
Objective To investigate the expression of microRNA-155 (miR-155) in peripheral B cells of the patients with systemic lupus erythematosus (SLE). Methods Sixty-six SLE patients and 10 healthy controls were enrolled in the study. The B cells were isolated from peripheral blood, and the expression of miR-155 in B cells was detected by fluorescence quantitative real-time RT-PCR (qRT-PCR). The levels of IgG in supernatants of B cells were determined by enzyme-linked immunosorbent assay (ELISA) after miR-155 expression was intervened by electrotransfection. Results The expression of miR-155 was significantly increased in B cells of the patients with SLE compared with healthy controls (P＜0.05). After intervention of the miR-155 expression with miRNA inhibitor, the levels of total IgG in the supernatants of the cultured B cells were significantly decreased. Conclusion The study indicates that the up-regulated miR-155 expression may be involved in the regulation of B cell function in systemic lupus erythematosus.关键词
系统性红斑狼疮/微小RNA/微小RNA-155/B细胞/IgGKey words
Systemic lupus erythematosus/microRNA/miR-155/B cell/IgG引用本文复制引用
陈阿琼,黄茜茜,叶璐璐,薛向阳,林巧爱,朱小春,李声东..系统性红斑狼疮患者B细胞中microRNA-155的表达及干预后效应[J].浙江医学,2018,40(10):1033-1036,1053,5.基金项目
宁波市科技局科研基金资助项目(2015A610179) （2015A610179）
