浙江医学2018，Vol.40Issue(10)：1037-1040,1053,后插1,6.DOI:10.12056/j.issn.1006-2785.2018.40.10.2018-101
山楂叶总黄酮对NAFLD肝细胞凋亡影响的研究
Effect of hawthorn leave flavonoids on apoptosis of hepatocyts in non-alcoholic fatty liver disease
摘要
Abstract
Objective To investigate the effects of hawthorn leave flavonoids (HLF) on apoptosis of hepatocytes in non-alcoholic fatty liver disease (NAFLD). Methods NAFLD model was induced in hepatic L-02 cells, the NAFLD cells were treated with 0, 100 or 400?g/ml HLF in model group, low dose HLF group and high dose HLF group, respectively. The morphological changes of cells were observed, the cell apoptosis was detected by flow cytometry, the expression of apoptosis-related proteins was examined by Western Blot. Results Compared to model group, the morphologic changes in HLF-treated groups were attenuated. The early apoptosis rate and middle/late apoptosis rate in high dose HLF group were significantly lower than those in model group (6.75±1.83％ vs.14.63 ± 1.82％,P＜0.05; 3.49 ± 1.03％ vs.11.36±2.94,P＜0.01). The middle/ late apoptosis rate of high dose HLF group was lower than that of low dose HLF group (3.49±1.03 ％ vs.8.79± 1.05％, P＜0.05). The overall apoptosis rate in the model group was significantly higher than that in HLF high and low dose groups, respectively (P＜0.01, P＜0.05). The expression of apoptosis-related proteins Bax, cytosolic cytochrome C (Cyt-C) and Caspase9 in the model group increased significantly, while and the expression of anti-apoptosis proteins Bcl-2, Mitochondria Cytochrome C (MCyt-C) was highly expressed in HLF high dose group and normal group. The ratio of Bax/Bcl-2 in the model group was significantly higher than those in HLF high and low dose groups (1.999 ±0.376 vs.0.144 ±0.023 and 0.399 ± 0.0454, respectively,P＜0.01). The ratio of Cytosolic /Mitochondria Cyt-C in the model group was significantly higher than those in HLF high and low dose groups (0.287±0.037 vs.0.041±0.006 and 0.097±0.024 respectively, P＜0.0I). The expression of t cleaved Caspase-9 in the model group was significantly higher than HLF high and low dose groups (0.060±0.005 vs.0.030±0.003 and 0.030 ±0.003 respectively, P＜0.01). Conclusion There is obvious cell apoptosis phenomenon in NAFLD, HLF can intervene apoptosis of hepatocytes through regulating the expression of Bcl-2, Bax, Cytochrome C, Caspase-9 and other apoptosis-related proteins.关键词
山楂叶总黄酮/非酒精性脂肪性肝病/凋亡/线粒体Key words
Hawthorn leaves flavonoids/Nonalcoholic fatty liver disease/Apoptosis/Mitochondria引用本文复制引用
黎运呈,曾芳,王秋景,郑迪,杨喆娟..山楂叶总黄酮对NAFLD肝细胞凋亡影响的研究[J].浙江医学,2018,40(10):1037-1040,1053,后插1,6.基金项目
浙江省中医药科技计划项目(2013ZQ029) （2013ZQ029）
