中南民族大学学报（自然科学版）2018，Vol.37Issue(2)：6-9,15,5.
生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP
Biotin Streptavidin System Combined Immunochromatography for Rapid Detection of NT-proBNP
摘要
Abstract
In order to improve the sensitivity of conventional immunochromatography,a novel rapid and quantitative assay for NT-proBNP was established by introducing biotin-streptavidin system and using fluorescent microspheres as a marker.The results showed that the new kit could detect NT-proBNP rapidly in the range of 0.02～30 μg·L－1.In whole blood test,the NT-proBNP concentration measured by the new test strip was in good agreement with the quantitative detection results of the RadioMeter AQT90 FLEX analyzer.The new immunochromatographic test strip greatly improved the accuracy of rapid test,and may be widely used in clinical diagnosis such as bedside test(POCTs).关键词
生物素-链霉亲和素/荧光免疫层析/N末端脑钠肽前体/即时检验Key words
biotin-streptavidin/fluorescence immunochromatography/NT-proBNP/POCTs分类
化学化工引用本文复制引用
孙宏浩,梁玉芬,赵晓双,黄玲,廖天作..生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP[J].中南民族大学学报（自然科学版）,2018,37(2):6-9,15,5.基金项目
国家自然科学基金资助项目(21501054,21401051) （21501054,21401051）
湖北省自然科学基金重点资助项目(2014CFA080) （2014CFA080）
湖北省教育厅优秀中青年科技创新团队(T201705) （T201705）
湖北省大学生创新创业训练计划资助项目(201410500021) （201410500021）
