中南民族大学学报（自然科学版）2018，Vol.37Issue(2)：24-30,7.
siRNA沉默DNA甲基化转移酶1基因干扰胎牛成纤维细胞增殖和凋亡
Interference of DNA Methyltransferase1Gene Silenced by siRNA on Fetal Bovine Fibroblast Cell Proliferation and Apoptosis
摘要
Abstract
To determine the effects of siRNAs targeting Dnmt1 on fetal bovine fibroblast cells(FBFCs),three siRNAs against Dnmt1 were designed for transfection into FBFCs.The results showed that Dnmt1-siRNA3 significantly reduced the mRNA expression level at 24,48 and 72 h(P＜0.01).At 48 h post-transfection,the inhibitory efficacy of siRNA3 against Dnmt1 reached nearly 80％.Dnmt1-siRNA3 treatment of FBFCs also significantly inhibited their proliferation,reduced cell viability and arrested more cells in the G0/G1transition(P＜0.05).However,Dnmt1-siRNA3 increased the number of apoptotic cells(P＜0.05).This study demonstrated that specific siRNAs targeting Dnmt1 effectively knocked down Dnmt1 mRNA expression in FBFCs,suggesting that the improved SCNT efficacy observed in the siRNA-treated donor cells might be partially due to changes in the cell cycle distribution.关键词
凋亡/细胞周期/供体细胞/增殖/RNA干扰Key words
apoptosis/cell cycle/donor cell/proliferation/RNA interference分类
生物科学引用本文复制引用
于孟飞,王文璐,易建明..siRNA沉默DNA甲基化转移酶1基因干扰胎牛成纤维细胞增殖和凋亡[J].中南民族大学学报（自然科学版）,2018,37(2):24-30,7.基金项目
国家自然科学基金青年资助项目(81400252) （81400252）
中央高校基本科研业务费专项资金资助项目(2013PY130) （2013PY130）
