中南民族大学学报（自然科学版）2018，Vol.37Issue(2)：49-53,5.
黄精多糖对环磷酰胺诱导小鼠免疫抑制的影响
Effects of Polygonatum sibiricum Polysaccharide on Immunosuppressive Action Induced by Cyclophosphamide in Mice
摘要
Abstract
Objective: To investigate the effects of Polygonatum sibiricum polysaccharide(PSP)on the immunosuppression activities induced by cyclophosphamide in mice.Methods: MTT assay was used to evaluate the proliferation activity of splenocytes of normal mice.Mice immunosuppression model was established with cyclophosphamide.The mice spleen,thymus index and spleen cell,thymocyte proliferation activity in vitro,macrophage phagocytosis,and the ability of macrophages to secrete inflammatory cytokines were respectively determined.Result: PSP could evidently increase the proliferation activity of spleen cell of normal mice.The synergistic proliferation promotion rate with ConA was more than 700％with PSP dose of 200 μg-mL－1.PSP could also improve the spleen and thymus index of immunosuppressed mice,promote the proliferation of spleen and thymus cells stimulated by ConA,and enhance the phagocytosis of macrophage and the secretion of IL-6 and TNF-α.Compared with the control group,the spleen index increased by 79.0％,43.3％in high PSP dose(150 mg-kg－1)for ConA synergized group,and the thymus indexes increased by 148.3％,48.5％respectively.Conclusion: PSP can significantly alleviate the immunosuppression of mice induced by cyclophosphamide.关键词
黄精多糖/环磷酰胺/免疫抑制Key words
Polygonatum sibiricum polysaccharide/cyclophosphamide/immunosuppression分类
生物科学引用本文复制引用
邓旭坤,段欢,刘钊,舒广文,江善青,余惠凡..黄精多糖对环磷酰胺诱导小鼠免疫抑制的影响[J].中南民族大学学报（自然科学版）,2018,37(2):49-53,5.基金项目
国家自然科学基金资助项目(81073147) （81073147）
国家科技部"十二·五"科技支撑计划资助项目(2012BAI27B06) （2012BAI27B06）
