作物学报2018，Vol.44Issue(7)：1000-1009,10.DOI:10.3724/SP.J.1006.2018.01000
玉米低磷胁迫诱导型强启动子P1502-ZmPHR1的克隆与表达分析
Cloning and Expression Analysis of Strong Inducible Promoter P1502-ZmPHR1 Responding to Low Phosphorus Stress in Maize
摘要
Abstract
ZmPHR1, a member of transcription factor MYB-CC family, enhanced phosphate absorption and improved plant growth when it was overexpressed under low-phosphate conditions. In order to elucidate the transcriptional regulatory mechanism of the promoter of ZmPHR1, its 5′upstream sequence P2205-ZmPHR1 was cloned from P-efficient maize inbred line 478. The full length of P2205-ZmPHR1 and four sequence deletion fragments in different lengths fused with the reporter gene GUS were trans-formed into Arabidopsis, respectively. The results of histochemical and quantitative fluorometric GUS assay showed that in the interval from 0 to -1502 bp, the response time and activity of sequence deletion fragments were increased with increasing frag-ment length. However, the activities were decreased or unchanged when the length of fragments were longer than 1502 bp. It was considered that the interval from 0 to -972 bp was function region and that from -972 bp to -1502 bp was function enhancing region. The results of qPCR analysis in transgenic plants at different time points (0, 1, 2, 3, and 4 days) after P-stress treatment showed that the activities of P1502-ZmPHR1 were influenced by both external signals and internal gene products, having feedback regulation. This study indicates that P1502-ZmPHR1 is a strong inducible promoter responding to low phosphorus stress and its functional activity is regulated by both external and internal phosphate concentration. These fundings provide an ideal promoter for crop improvement under low phosphate conditions and a basis for its application.关键词
玉米/低磷胁迫/强诱导型启动子/P1502-ZmPHR1Key words
maize/low phosphorus stress/strong inducible promoter/P1502-ZmPHR1引用本文复制引用
杨瑞娟,白建荣,闫蕾,苏亮,王秀红,李锐,张丛卓..玉米低磷胁迫诱导型强启动子P1502-ZmPHR1的克隆与表达分析[J].作物学报,2018,44(7):1000-1009,10.基金项目
本研究由山西省农业科学院农业科技创新项目(ZDSYS1507)资助.This study was supported by the Agricultural Science and Technology Innovation Program of Shanxi Academy of Agricultural Sciences (ZDSY1507). （ZDSYS1507）
