作物学报2018，Vol.44Issue(7)：1095-1102,8.DOI:10.3724/SP.J.1006.2018.01095
四倍体马铃薯块茎蛋白含量分子标记的开发与验证
Development and Validation of Molecular Marker for Protein Content in Tetraploid Potato Tuber
摘要
Abstract
Protein content is one of the most important quality traits in potato. In this study, SCAR8-107, a protein content linked molecular marker, was developed using Atlantic, Dingshu 1, and their F1segregation population consisting of 173 individuals by reduced-representation sequencing and bulked segregant analysis. A further testing of marker SCAR8-107 was performed using 74 high protein content individuals, 42 low protein content individuals and some tetraploid varieties, showing the selection accu-racy of 90.51% and 72.97% between marker test and phenotyping in segregation population and tetraploid varieties respectively. Pearson’s correlation analysis also showed that the marker SCAR8-107 was significantly correlated to protein content in potato. The SCAR8-107 marker could be used in marker-assisted selection.关键词
马铃薯蛋白/标记开发/BSA混池测序/分子标记辅助育种Key words
potato protein/marker development/BSA mixing pool sequencing/marker-assisted selection引用本文复制引用
单洪波,史佳文,石瑛..四倍体马铃薯块茎蛋白含量分子标记的开发与验证[J].作物学报,2018,44(7):1095-1102,8.基金项目
本研究由国家重点研发计划项目(2017YFD0101906)资助.This study was supported by the National Key Research and Development Programs (2017YFD0101906). （2017YFD0101906）
