中药材2017，Vol.40Issue(11)：2590-2593,4.DOI:10.13863/j.issn1001-4454.2017.11.021
黄边灵芝麦角甾类化学成分及其细胞毒活性研究
Study on Ergostane Steroids of Ganoderma luteomarginatum and Their Cytotoxicities
摘要
Abstract
To study the chemical constituents from the sporophore of Ganoderma luteomarginatum and their cytotoxic activities. Methods: The compounds were separated and repeatedly purified by column chromatography over silica gel and Sephadex LH-20,preparative TLC, and semi-preparative HPLC. Their structures were elucidated by NMR and MS spectroscopic methods. Furthermore, the cytotoxicities of the chemical components for mouse colorectal carcinoma CT26 cell line were measured by MTT method. Results: Seven ergostane-type steroids were isolated from Ganoderma luteomarginatum, and their structures were identified as 5α,8α-epidioxyergosta-6,22-dien-3β-ol (1),5α,8α-epidioxyergosta-6,9 (11),22-triene-3β-ol (2),ergosterol (3),ergosta-7,22-dien-3β-ol(4), ergosta-7, 22-dien-3-one(5), calvasterol A(6) and volemolide(7). Compounds 1～ 5 inhibited the growth of CT26 lines with IC50 values of 76. 93, 37. 19, 13. 02, 48. 19, and 1. 25 μmol/L, respectively. Conclusion: All compounds are isolated from Ganoderma luteomarginatum for the first time, compounds 6 and 7 are first reported from the Ganoderma genus, and compounds 1～ 5 show significant cytotoxic activities against mouse colorectal carcinoma CT26 cell line.关键词
黄边灵芝/麦角甾类成分/结构鉴定/细胞毒活性Key words
Ganoderma luteomarginatum J. D. Zhao,L. W. Hsu et X. Q. Zhang/Ergostane-type steroids/Structure identification/Cytotoxic activity分类
医药卫生引用本文复制引用
苏海国,何育霖,熊亮,王月,郭力,戴鸥..黄边灵芝麦角甾类化学成分及其细胞毒活性研究[J].中药材,2017,40(11):2590-2593,4.基金项目
四川省青年科技创新研究团队专项计划项目(2016TD0006,2017TD0001) （2016TD0006,2017TD0001）
