中药材2017，Vol.40Issue(11)：2607-2611,5.DOI:10.13863/j.issn1001-4454.2017.11.025
雌、雄地鳖中17种氨基酸的含量测定及指纹图谱研究
Study on HPLC Fingerprint and Determine Amino Acids in Female and Male Eupolyphaga sinensis
何新荣 1古今 1朱旻 1孟庆隆 1傅深盈 1孙艳1
作者信息
- 1. 中国人民解放军总医院药学部,北京 100853
- 折叠
摘要
Abstract
To establish the fingerprint of free amino acids in Eupolyphaga sinensis,and to study the difference of the content of various amino acids in female and male Eupolyphaga sinensis. Methods: The free amino acids were extracted by ultrasonic in 0. 1 mol/L HCl. O-Phthalaldehyde(OPA) was used as the pre-column derivation reagent, and the content of amino acids was determined by HPLC method. Agilent ZOBAX SB-C18 (250 mm × 4. 6 mm,5 μm) was used as chromatographic column,mobile phase A was sodium phosphate buffer(0. 1 mol/L,pH= 7. 5),mobile phase B was methanol-acetonitrile-water(45∶45∶10); the excitation wavelength was set at 340 nm and emission wavelength was set at 450 nm, the flow rate was 1. 0 mL/min in gradient elution, and the column temperature was 30 ℃. Results: The fingerprint of free amino acids in Eupolyphaga sinensis was established, 21 common peaks were calibrated in the fingerprint and 17 amino acids contents were quantitatively analysed. The similarities of 15 batches of female Eupolyphaga sinensis were no less than 0. 97,which analyzed by"Similarity Evaluation System for Chromatographic Fingerprint of TMC"edited by China Pharmacopoeia Committee. The content of amino acids was large different in different batches of Eupolyphaga sinensis samples, and the content of amino acids of Eupolyphaga sinensis in Hubei province was higher than that in Jiangsu province and Hebei province, and the content of total free amino acids in female Eupolyphaga sinensis was higher than that in males Eupolyphaga sinensis. Conclusion: The method is accurate and simple, and thereby can be used specifically for the quality control of Eupolyphaga sinensis. There are differences content of the free amino acid between in female and male Eupolyphaga sinensis,which can provide new scientific basis for female Eupolyphaga sinensis for its medical use.关键词
地鳖/氨基酸/柱前衍生/HPLC/指纹图谱Key words
Eupolyphaga sinensis Walker/Amino acids/Pre-column derivatization/HPLC/Fingerprint分类
医药卫生引用本文复制引用
何新荣,古今,朱旻,孟庆隆,傅深盈,孙艳..雌、雄地鳖中17种氨基酸的含量测定及指纹图谱研究[J].中药材,2017,40(11):2607-2611,5.
