中国现代中药2018，Vol.20Issue(6)：658-662,5.DOI:10.13313/j.issn.1673-4890.20171227007
SCoT分子标记对不同产地白芍亲缘关系的研究
Study on the Genetic Relationship of Paeonia lactiflora from Different Regions by SCoT Molecular Markers
摘要
Abstract
Objective:This study used SCoT molecular marker technique to find the relationship between the 22 groups of Paeoniae lactiflora samples,which were distributed in the Zhejiang panan,Anhui haozhou,Sichuan zhongjiang and Shandong heze.Methods:Extracted Paeonia lactiflora DNA,Optimal PCR reaction system and found the best suitable for SCoT molecular marker of P.lactiflora by orthogonal design.Screened the SCoT primers obtained from PCR amplification.Finally,applied the NTSYS2.10e software and the UPGMA method was used for cluster analysis.Results:Fin-ded that SCoT primer sequence amplificated out 121 bands,included 77 polymorphism bands,polymorphic percentage was 63.6%, the varieties of similarity coefficient between 0.78 ～ 0.94,P.lactiflora of different regions were respectively divided into category.Conclusion:The results showed that there was a rich genetic polymorphism of P.lactiflora in different habitats,and the SCoT molecular marker was suitable for the study of the relationship between P.lactiflora from different sources.The effective differ-entiation of P.lactiflora varieties from different habitats provided a strong reference for the cultivation and standardization of germ-plasm resources of P.lactiflora.关键词
SCoT/白芍/正交/遗传多态性Key words
SCoT/Paeoniae lactiflora/orthogonal/genetic polymorphism引用本文复制引用
席秀利,胡珊,邬龙怡,钟旺琴..SCoT分子标记对不同产地白芍亲缘关系的研究[J].中国现代中药,2018,20(6):658-662,5.基金项目
广东省中药(香雪)工程技术研究中心(2015S-P095) （香雪）
